Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | sprG-sprF/- |
| Location | 563426..563596 | Replicon | chromosome |
| Accession | NZ_JPPU01000009 | ||
| Organism | Staphylococcus aureus strain HG003 isolate RN1 Scaffold_9 | ||
Toxin (Protein)
| Gene name | SprG4 | Uniprot ID | - |
| Locus tag | - | Protein ID | - |
| Coordinates | 563543..563596 (-) | Length | 18 a.a. |
Antitoxin (RNA)
| Gene name | SprF4 | ||
| Locus tag | - | ||
| Coordinates | 563426..563575 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IM26_RS23595 (IM26_08750) | 559104..559391 | - | 288 | WP_000410720.1 | hypothetical protein | - |
| IM26_RS28755 (IM26_08755) | 559670..559960 | + | 291 | WP_001795544.1 | hypothetical protein | - |
| IM26_RS23605 (IM26_08760) | 560048..560689 | - | 642 | WP_000571178.1 | ABC transporter ATP-binding protein | - |
| IM26_RS23610 (IM26_08765) | 560686..561006 | - | 321 | WP_000668623.1 | YxeA family protein | - |
| IM26_RS23615 (IM26_08770) | 561009..562973 | - | 1965 | WP_000870806.1 | bacteriocin-associated integral membrane family protein | - |
| IM26_RS28760 | 563017..563277 | - | 261 | WP_130137088.1 | lactococcin 972 family bacteriocin | - |
| IM26_RS0114625 | 563299..563400 | - | 102 | WP_001790623.1 | hypothetical protein | - |
| - | 563426..563575 | + | 150 | - | - | Antitoxin |
| - | 563543..563596 | - | 54 | - | - | Toxin |
| IM26_RS0114630 | 563946..564065 | - | 120 | WP_020978111.1 | hypothetical protein | - |
| IM26_RS23620 (IM26_08780) | 564317..564919 | - | 603 | WP_001033867.1 | CPBP family glutamic-type intramembrane protease | - |
| IM26_RS0114635 | 564934..565110 | - | 177 | WP_000214898.1 | YkvS family protein | - |
| IM26_RS23625 (IM26_08790) | 565309..566295 | + | 987 | WP_000668820.1 | lipoate--protein ligase | - |
| IM26_RS23630 (IM26_08795) | 566376..566594 | - | 219 | WP_000876826.1 | IDEAL domain-containing protein | - |
| IM26_RS23635 (IM26_08800) | 566804..567373 | + | 570 | WP_000287265.1 | competence protein ComK | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 18 a.a. Molecular weight: 2150.39 Da Isoelectric Point: 5.9481
>T10148 - NZ_JPPU01000009:c563596-563543 [Staphylococcus aureus]
MINLNNKKQPSFPNFDNE
MINLNNKKQPSFPNFDNE
Download Length: 54 bp
>T10148 NZ_JPPU01000009:c563596-563543 [Staphylococcus aureus]
ATGATTAATTTAAACAATAAAAAACAACCATCATTTCCAAACTTTGACAACGAA
ATGATTAATTTAAACAATAAAAAACAACCATCATTTCCAAACTTTGACAACGAA
Antitoxin
Download Length: 150 bp
>AT10148 NZ_JPPU01000009:563426-563575 [Staphylococcus aureus]
AGTATAATTGTATTATTATTAAATTGTGCAGTGTATAAATAGACGATATGCAGGAACATGTCGCCTATTGAGCCCGTTAA
AAAGACGGTGACTAAATGAGATTTTCTTTAACCATCATTCGTTGTCAAAGTTTGGAAATGATGGTTGTTT
AGTATAATTGTATTATTATTAAATTGTGCAGTGTATAAATAGACGATATGCAGGAACATGTCGCCTATTGAGCCCGTTAA
AAAGACGGTGACTAAATGAGATTTTCTTTAACCATCATTCGTTGTCAAAGTTTGGAAATGATGGTTGTTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10027 | Staphylococcus aureus subsp. aureus N315 |
94.444 |
100 |
0.944 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Camille Riffaud et al. (2019) Functionality and cross-regulation of the four SprG/SprF type I toxin-antitoxin systems in Staphylococcus aureus. Nucleic Acids Research 47(4):1740-1758. [PubMed:30551143]