Detailed information of TA system TA101472

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52398..52819
• Replicon: plasmid pCTXM15_005784
• Accession: NZ_CP028576
• Organism: Escherichia coli strain WCHEC005784

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: DBQ99_RS00445
• Protein ID: WP_001302184.1
• Coordinates: 52398..52556 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	52621..52819 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBQ99_RS00410	47462..47677	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
DBQ99_RS00415	47813..48460	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
DBQ99_RS00420	48651..49034	-	384	WP_001063020.1	relaxosome protein TraM	-
DBQ99_RS00425	49404..49976	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
DBQ99_RS00430	50272..51093	-	822	WP_001234465.1	DUF945 domain-containing protein	-
DBQ99_RS00435	51212..51499	-	288	WP_000107242.1	hypothetical protein	-
DBQ99_RS26330	51524..51730	-	207	WP_000275854.1	hypothetical protein	-
DBQ99_RS00445	52398..52556	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	52621..52819	-	199	NuclAT_0	-	Antitoxin
-	52621..52819	-	199	NuclAT_0	-	Antitoxin
-	52621..52819	-	199	NuclAT_0	-	Antitoxin
-	52621..52819	-	199	NuclAT_0	-	Antitoxin
DBQ99_RS00450	52788..52893	-	106	Protein_75	plasmid SOS inhibition protein A	-
DBQ99_RS00455	53048..53365	+	318	Protein_76	IS110 family transposase	-
DBQ99_RS00460	53654..53905	-	252	Protein_77	iron-containing alcohol dehydrogenase	-
DBQ99_RS00465	53990..54613	-	624	WP_000912556.1	potassium channel family protein	-
DBQ99_RS00470	54629..55326	-	698	Protein_79	IS1 family transposase	-
DBQ99_RS00480	55383..55487	+	105	Protein_80	thiosulfate reductase cytochrome B subunit	-
DBQ99_RS00490	55733..57127	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-15 / aac(3)-IId / blaTEM-1B / blaOXA-1 / aac(6')-Ib-cr / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	-	1..112422	112422
	-	flank	IS/Tn	-	-	54629..55132	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T101472 WP_001302184.1 NZ_CP028576:c52556-52398 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T101472 NZ_CP028576:c52556-52398 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT101472 NZ_CP028576:c52819-52621 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin