Detailed information of TA system TA101470

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30953..31207
• Replicon: plasmid pCTXM15_005784
• Accession: NZ_CP028576
• Organism: Escherichia coli strain WCHEC005784

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: DBQ99_RS00260
• Protein ID: WP_032215358.1
• Coordinates: 30953..31159 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	31146..31207 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBQ99_RS00220	26974..27375	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
DBQ99_RS26050	27308..27565	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
DBQ99_RS00225	27658..28311	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
DBQ99_RS00240	29252..30109	-	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
DBQ99_RS00245	30102..30176	-	75	WP_001365705.1	RepA leader peptide Tap	-
DBQ99_RS00255	30412..30669	-	258	WP_000083833.1	replication regulatory protein RepA	-
DBQ99_RS00260	30953..31159	-	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	31146..31207	+	62	NuclAT_1	-	Antitoxin
-	31146..31207	+	62	NuclAT_1	-	Antitoxin
-	31146..31207	+	62	NuclAT_1	-	Antitoxin
-	31146..31207	+	62	NuclAT_1	-	Antitoxin
DBQ99_RS26325	31346..31612	-	267	WP_087757657.1	hypothetical protein	-
DBQ99_RS00275	31987..32556	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
DBQ99_RS00280	32708..33334	-	627	WP_029702149.1	hypothetical protein	-
DBQ99_RS00285	33746..33955	-	210	WP_039023209.1	hypothetical protein	-
DBQ99_RS00290	34086..34646	-	561	WP_001567328.1	fertility inhibition protein FinO	-
DBQ99_RS00295	34701..34937	-	237	Protein_45	conjugal transfer protein	-
DBQ99_RS00305	35025..35528	-	504	Protein_46	conjugal transfer protein TraH	-
DBQ99_RS00310	35515..35940	-	426	WP_071594227.1	F-type conjugal transfer protein TrbF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-15 / aac(3)-IId / blaTEM-1B / blaOXA-1 / aac(6')-Ib-cr / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	-	1..112422	112422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T101470 WP_032215358.1 NZ_CP028576:c31159-30953 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T101470 NZ_CP028576:c31159-30953 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT101470 NZ_CP028576:31146-31207 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin