Detailed information of TA system TA101464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 71119..71770
• Replicon: plasmid p1_005046
• Accession: NZ_CP028569
• Organism: Acinetobacter pittii strain WCHAP005046

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: DBQ26_RS00445
• Protein ID: WP_006581604.1
• Coordinates: 71119..71475 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBQ26_RS00450
• Protein ID: WP_001140619.1
• Coordinates: 71468..71770 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBQ26_RS00425	66583..67011	+	429	WP_002124576.1	hypothetical protein	-
DBQ26_RS00430	66936..67136	+	201	Protein_82	GABA permease	-
DBQ26_RS00435	67537..69645	+	2109	WP_002124575.1	TonB-dependent siderophore receptor	-
DBQ26_RS00440	70090..70518	-	429	WP_000877730.1	hypothetical protein	-
DBQ26_RS00445	71119..71475	+	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBQ26_RS00450	71468..71770	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
DBQ26_RS00455	71763..71972	+	210	WP_000069471.1	hypothetical protein	-
DBQ26_RS00460	72082..72300	-	219	WP_029747731.1	hypothetical protein	-
DBQ26_RS00470	72924..73091	+	168	WP_002072926.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DBQ26_RS00475	73078..73367	+	290	Protein_90	helix-turn-helix domain-containing protein	-
DBQ26_RS00480	73631..74790	+	1160	WP_085945698.1	IS3 family transposase	-
DBQ26_RS00495	75157..75462	-	306	Protein_92	hypothetical protein	-
DBQ26_RS00500	75518..76450	+	933	WP_005063910.1	IS5 family transposase	-
DBQ26_RS00505	76454..76672	-	219	WP_107908570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..121612	121612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T101464 WP_006581604.1 NZ_CP028569:71119-71475 [Acinetobacter pittii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T101464 NZ_CP028569:71119-71475 [Acinetobacter pittii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGACCTACGTGTTCAGCATCGAGGTAAATCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGTGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT101464 WP_001140619.1 NZ_CP028569:71468-71770 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT101464 NZ_CP028569:71468-71770 [Acinetobacter pittii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAATTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0