Detailed information of TA system TA101455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 165989..166574
• Replicon: plasmid pNDM1_010045
• Accession: NZ_CP028560
• Organism: Acinetobacter sp. WCHA45

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: CDG55_RS01000
• Protein ID: WP_000897307.1
• Coordinates: 166254..166574 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDG55_RS00995
• Protein ID: WP_000369781.1
• Coordinates: 165989..166261 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG55_RS00970	161567..162204	-	638	Protein_159	transposase	-
CDG55_RS00975	162283..162828	-	546	Protein_160	helix-turn-helix domain-containing protein	-
CDG55_RS00985	163080..163964	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
CDG55_RS00990	164020..165495	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
CDG55_RS00995	165989..166261	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
CDG55_RS01000	166254..166574	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDG55_RS01005	166701..168026	-	1326	WP_001280601.1	ISNCY family transposase	-
CDG55_RS01015	168162..168710	-	549	Protein_166	LysE family transporter	-
CDG55_RS01020	168737..169827	+	1091	WP_108565324.1	IS4-like element ISAba33 family transposase	-
CDG55_RS01030	170006..170833	-	828	WP_000073658.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / blaNDM-1 / sul2 / floR / mph(E) / msr(E)	-	1..190170	190170
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	161561..183137	21576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T101455 WP_000897307.1 NZ_CP028560:c166574-166254 [Acinetobacter sp. WCHA45]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T101455 NZ_CP028560:c166574-166254 [Acinetobacter sp. WCHA45]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT101455 WP_000369781.1 NZ_CP028560:c166261-165989 [Acinetobacter sp. WCHA45]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT101455 NZ_CP028560:c166261-165989 [Acinetobacter sp. WCHA45]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3