Detailed information of TA system TA101454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 125109..125760
• Replicon: plasmid pNDM1_010045
• Accession: NZ_CP028560
• Organism: Acinetobacter sp. WCHA45

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CDG55_RS00830
• Protein ID: WP_087537454.1
• Coordinates: 125404..125760 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDG55_RS00825
• Protein ID: WP_001140620.1
• Coordinates: 125109..125411 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG55_RS00795	120176..121501	-	1326	WP_087537514.1	ISNCY family transposase	-
CDG55_RS00805	121769..123049	-	1281	WP_087537516.1	cation diffusion facilitator family transporter	-
CDG55_RS00810	123139..123531	+	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
CDG55_RS00815	123625..123786	+	162	Protein_128	IS6 family transposase	-
CDG55_RS00820	123912..125066	+	1155	WP_005003123.1	IS4 family transposase	-
CDG55_RS00825	125109..125411	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
CDG55_RS00830	125404..125760	-	357	WP_087537454.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDG55_RS00835	126516..128171	+	1656	WP_087537455.1	HNH endonuclease	-
CDG55_RS00840	128723..129955	+	1233	WP_087537456.1	ATP-binding protein	-
CDG55_RS00845	130227..130556	-	330	WP_000506885.1	DUF4236 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / blaNDM-1 / sul2 / floR / mph(E) / msr(E)	-	1..190170	190170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13652.72 Da
• Isoelectric Point: 7.1654

>T101454 WP_087537454.1 NZ_CP028560:c125760-125404 [Acinetobacter sp. WCHA45]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQVPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKEMLDIADQQYELHLSTLGDKSNG

Nucleotide

• Length: 357 bp

>T101454 NZ_CP028560:c125760-125404 [Acinetobacter sp. WCHA45]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAACCTACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGTACCGAGTTTAGGCCGTCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAGTACGAACT
TCATCTTTCAACTTTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT101454 WP_001140620.1 NZ_CP028560:c125411-125109 [Acinetobacter sp. WCHA45]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT101454 NZ_CP028560:c125411-125109 [Acinetobacter sp. WCHA45]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA