Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4029034..4029631 Replicon chromosome
Accession NZ_CP028555
Organism Klebsiella variicola strain WCHKP19

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag CAY66_RS23765 Protein ID WP_087498842.1
Coordinates 4029314..4029631 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CAY66_RS23760 Protein ID WP_012542525.1
Coordinates 4029034..4029321 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAY66_RS23730 4024938..4025186 + 249 WP_008805539.1 DUF1158 domain-containing protein -
CAY66_RS23735 4025203..4025544 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
CAY66_RS23740 4025575..4026690 - 1116 WP_162499993.1 MBL fold metallo-hydrolase -
CAY66_RS23745 4026870..4027451 + 582 WP_012968754.1 TetR/AcrR family transcriptional regulator -
CAY66_RS23750 4027451..4027819 + 369 WP_008805536.1 MmcQ/YjbR family DNA-binding protein -
CAY66_RS23755 4027939..4028592 + 654 WP_012968755.1 oxygen-insensitive NAD(P)H nitroreductase -
CAY66_RS23760 4029034..4029321 - 288 WP_012542525.1 helix-turn-helix transcriptional regulator Antitoxin
CAY66_RS23765 4029314..4029631 - 318 WP_087498842.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CAY66_RS23770 4029816..4030859 - 1044 WP_016160437.1 DUF2157 domain-containing protein -
CAY66_RS23775 4031389..4032072 - 684 Protein_3848 helix-turn-helix domain-containing protein -
CAY66_RS23780 4032102..4033901 + 1800 WP_052455150.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-89)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12076.37 Da        Isoelectric Point: 11.2767

>T101447 WP_087498842.1 NZ_CP028555:c4029631-4029314 [Klebsiella variicola]
MLRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T101447 NZ_CP028555:c4029631-4029314 [Klebsiella variicola]
ATGTTAAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10815.51 Da        Isoelectric Point: 10.2212

>AT101447 WP_012542525.1 NZ_CP028555:c4029321-4029034 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Download         Length: 288 bp

>AT101447 NZ_CP028555:c4029321-4029034 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References