Detailed information of TA system TA101407

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72695..72948
• Replicon: plasmid pKPC2_020143
• Accession: NZ_CP028547
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain SCKP020143

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CLQ46_RS02170
• Protein ID: WP_001312851.1
• Coordinates: 72799..72948 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	72695..72754 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ46_RS02125	68355..68420	-	66	Protein_92	hypothetical protein	-
CLQ46_RS02130	68473..69177	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CLQ46_RS02135	69241..69402	+	162	Protein_94	DNA helicase	-
CLQ46_RS02140	69422..70168	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
CLQ46_RS02145	70223..70783	+	561	WP_000139328.1	fertility inhibition protein FinO	-
CLQ46_RS02150	70915..71115	+	201	WP_015059022.1	hypothetical protein	-
CLQ46_RS02155	71501..72100	+	600	WP_032083981.1	hypothetical protein	-
CLQ46_RS02160	72264..72494	+	231	WP_001736714.1	hypothetical protein	-
CLQ46_RS02165	72517..72744	-	228	Protein_100	hypothetical protein	-
-	72695..72754	-	60	NuclAT_0	-	Antitoxin
-	72695..72754	-	60	NuclAT_0	-	Antitoxin
-	72695..72754	-	60	NuclAT_0	-	Antitoxin
-	72695..72754	-	60	NuclAT_0	-	Antitoxin
CLQ46_RS02170	72799..72948	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
CLQ46_RS02175	73232..73480	+	249	WP_000083837.1	replication regulatory protein RepA	-
CLQ46_RS02180	73481..73690	-	210	WP_064765370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / rmtB / blaKPC-2 / blaSHV-12 / catA2	-	1..73791	73791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T101407 WP_001312851.1 NZ_CP028547:72799-72948 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T101407 NZ_CP028547:72799-72948 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT101407 NZ_CP028547:c72754-72695 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin