Detailed information of TA system TA101382

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 176420..176673
• Replicon: plasmid pKPC2_020002
• Accession: NZ_CP028541
• Organism: Klebsiella pneumoniae strain WCHKP2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CAY63_RS01430
• Protein ID: WP_001312851.1
• Coordinates: 176524..176673 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	176420..176479 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY63_RS01385	172080..172145	-	66	Protein_252	recombinase family protein	-
CAY63_RS01390	172198..172902	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CAY63_RS01395	172966..173127	+	162	Protein_254	DNA helicase	-
CAY63_RS01400	173147..173893	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
CAY63_RS01405	173948..174508	+	561	WP_000139328.1	fertility inhibition protein FinO	-
CAY63_RS01410	174640..174840	+	201	WP_015059022.1	hypothetical protein	-
CAY63_RS01415	175226..175825	+	600	WP_032083981.1	hypothetical protein	-
CAY63_RS01420	175989..176219	+	231	WP_001736714.1	hypothetical protein	-
CAY63_RS01425	176242..176469	-	228	Protein_260	hypothetical protein	-
-	176420..176479	-	60	NuclAT_1	-	Antitoxin
-	176420..176479	-	60	NuclAT_1	-	Antitoxin
-	176420..176479	-	60	NuclAT_1	-	Antitoxin
-	176420..176479	-	60	NuclAT_1	-	Antitoxin
CAY63_RS01430	176524..176673	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
CAY63_RS01435	176957..177205	+	249	WP_000083837.1	replication regulatory protein RepA	-
CAY63_RS01440	177206..177415	-	210	WP_064765370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..177516	177516

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T101382 WP_001312851.1 NZ_CP028541:176524-176673 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T101382 NZ_CP028541:176524-176673 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT101382 NZ_CP028541:c176479-176420 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin