Detailed information of TA system TA101343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 118396..119027
• Replicon: plasmid unnamed1
• Accession: NZ_CP028488
• Organism: Yersinia massiliensis strain GTA

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2R4NX45
• Locus tag: DA391_RS23765
• Protein ID: WP_049615238.1
• Coordinates: 118396..118686 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DA391_RS23770
• Protein ID: WP_049615233.1
• Coordinates: 118698..119027 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA391_RS23745	114484..115122	-	639	WP_108088347.1	DUF1120 domain-containing protein	-
DA391_RS23750	115422..116117	+	696	Protein_115	IS6 family transposase	-
DA391_RS23755	116170..117045	+	876	Protein_116	ISL3 family transposase	-
DA391_RS23760	117533..118258	+	726	WP_159074589.1	N-6 DNA methylase	-
DA391_RS23765	118396..118686	+	291	WP_049615238.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DA391_RS23770	118698..119027	+	330	WP_049615233.1	HigA family addiction module antidote protein	Antitoxin
DA391_RS23775	119433..120038	-	606	Protein_120	transposase	-
DA391_RS23780	120037..120258	+	222	Protein_121	Tn3 family transposase	-
DA391_RS23785	120324..120617	-	294	Protein_122	transposase zinc-binding domain-containing protein	-
DA391_RS23790	120700..121086	-	387	Protein_123	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..121622	121622
	-	inside	IScluster/Tn	-	-	115422..121086	5664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (7-85)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11538.18 Da
• Isoelectric Point: 9.3579

>T101343 WP_049615238.1 NZ_CP028488:118396-118686 [Yersinia massiliensis]
MIKSFRDKNLELFYMESKRDRAIPATIERQLAKKLDMLAAAHNERDLFIPTSNYYKRLSGQFDGWSSMRVNIQWRLMFQW
KDGYAESIYLDPHQDT

Nucleotide

• Length: 291 bp

>T101343 NZ_CP028488:118396-118686 [Yersinia massiliensis]
ATGATTAAGTCTTTTCGGGATAAAAACCTAGAACTGTTCTATATGGAAAGTAAACGAGATCGCGCGATACCGGCAACAAT
AGAGCGCCAGTTAGCGAAGAAGCTTGATATGTTGGCCGCCGCACATAATGAGCGAGATTTGTTTATTCCAACCAGTAATT
ACTACAAGCGTCTCTCAGGGCAGTTTGATGGCTGGTCAAGTATGAGAGTCAATATTCAATGGCGGTTAATGTTTCAATGG
AAAGACGGATATGCTGAAAGCATCTACCTCGATCCTCATCAAGACACCTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12630.54 Da
• Isoelectric Point: 6.2374

>AT101343 WP_049615233.1 NZ_CP028488:118698-119027 [Yersinia massiliensis]
MLDTTTREPTTVGEILQEEFLTPLAIGQEELADIMGVTRATVNRLCKGRRRLTVEEATLFSELFETTPEFWLNLQAAHDR
WEAREALQKHKRPPMRPIMEMLGRRISHA

Nucleotide

• Length: 330 bp

>AT101343 NZ_CP028488:118698-119027 [Yersinia massiliensis]
ATGCTTGATACCACTACTCGCGAACCTACTACGGTAGGCGAAATTTTGCAGGAAGAATTTTTAACCCCATTAGCCATTGG
GCAGGAAGAACTGGCCGATATTATGGGCGTGACTCGCGCAACGGTTAACCGTTTGTGCAAAGGCCGTCGTCGTCTGACTG
TAGAAGAAGCGACGCTGTTTTCTGAGTTGTTTGAGACGACGCCCGAATTTTGGTTGAACCTCCAAGCCGCTCATGACCGT
TGGGAAGCACGAGAAGCGCTTCAAAAACATAAGCGACCCCCAATGCGTCCTATCATGGAAATGTTGGGGCGGCGTATCTC
ACACGCTTGA

Structures

Toxin

• AlphaFold DB: A0A2R4NX45