Detailed information of TA system TA101342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 39826..40571
• Replicon: plasmid unnamed1
• Accession: NZ_CP028488
• Organism: Yersinia massiliensis strain GTA

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DA391_RS23350
• Protein ID: WP_088130822.1
• Coordinates: 39826..40137 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DA391_RS23355
• Protein ID: WP_088130831.1
• Coordinates: 40137..40571 (+)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DA391_RS23325	35534..36355	+	822	WP_088130827.1	N-6 DNA methylase	-
DA391_RS23330	36874..37374	+	501	WP_088130826.1	hypothetical protein	-
DA391_RS23335	37600..38577	+	978	WP_088130825.1	StbA family protein	-
DA391_RS23340	38584..38988	+	405	WP_088130824.1	plasmid partitioning/stability family protein	-
DA391_RS23345	39088..39651	+	564	WP_088130823.1	helix-turn-helix transcriptional regulator	-
DA391_RS23350	39826..40137	+	312	WP_088130822.1	type II toxin-antitoxin system HigB family toxin	Toxin
DA391_RS23355	40137..40571	+	435	WP_088130831.1	helix-turn-helix domain-containing protein	Antitoxin
DA391_RS24285	41092..41304	+	213	WP_159074585.1	hypothetical protein	-
DA391_RS23365	41532..42461	+	930	WP_159074586.1	DUF1281 domain-containing protein	-
DA391_RS24395	42720..42938	-	219	WP_176396509.1	hypothetical protein	-
DA391_RS23375	42923..43423	+	501	WP_167398252.1	antirestriction protein ArdA	-
DA391_RS23380	43467..43670	+	204	WP_088130818.1	hypothetical protein	-
DA391_RS23390	44350..44631	+	282	WP_088130816.1	ribulokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..121622	121622

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (87-137)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12503.41 Da
• Isoelectric Point: 10.1438

>T101342 WP_088130822.1 NZ_CP028488:39826-40137 [Yersinia massiliensis]
MRVISKKPFDEAGKLFPNDAAALDDIYKVLHTERFLTPDHLKARFRSLDRFKYRQKWWVIDIGGNNLRIIAYIKFETQNV
FIKHIVTHKEYDRLTDYYRRTPE

Nucleotide

• Length: 312 bp

>T101342 NZ_CP028488:39826-40137 [Yersinia massiliensis]
ATGCGGGTTATTTCAAAGAAACCGTTTGATGAGGCGGGGAAGTTATTTCCGAATGATGCTGCGGCGCTAGATGATATTTA
TAAAGTGCTGCATACCGAGCGGTTTTTAACGCCTGATCATCTTAAAGCGCGTTTTCGAAGTTTGGACAGATTCAAATACA
GACAAAAATGGTGGGTGATAGATATCGGTGGGAACAATTTACGAATAATCGCCTATATAAAGTTTGAAACTCAGAACGTG
TTTATTAAGCACATTGTGACTCACAAAGAATATGACAGGCTCACCGATTACTACCGGAGAACTCCAGAATGA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 16004.29 Da
• Isoelectric Point: 4.4959

>AT101342 WP_088130831.1 NZ_CP028488:40137-40571 [Yersinia massiliensis]
MTTNSYEVALNATAALINAVPLLGGSHSREDYEQALEMVERLIDTDDENPLIDLLAKKIADYENTAPEFEAFNARIAALP
QELAMLRVIMDQHGLNQSSFKEEIGARSLVSMILKGERKLTLEHMKALSKRFGLPVSAFIDENV

Nucleotide

• Length: 435 bp

>AT101342 NZ_CP028488:40137-40571 [Yersinia massiliensis]
ATGACAACAAACAGTTATGAGGTTGCTTTAAATGCGACGGCCGCATTAATAAATGCCGTGCCCTTGTTGGGAGGGAGCCA
TAGCCGTGAAGACTACGAGCAGGCTCTTGAGATGGTAGAACGCCTAATCGATACTGACGACGAGAACCCGCTGATCGACC
TGTTGGCTAAAAAAATTGCTGATTATGAAAATACTGCCCCCGAATTCGAGGCGTTCAACGCGCGTATAGCTGCATTACCG
CAAGAGTTGGCGATGCTACGCGTGATAATGGATCAGCACGGGCTAAATCAAAGTAGCTTCAAAGAGGAAATTGGTGCCCG
TTCTTTGGTCAGTATGATCTTGAAAGGAGAGCGTAAGCTGACTCTGGAGCACATGAAAGCTCTTTCAAAGCGCTTTGGAC
TACCTGTTTCTGCCTTTATTGATGAAAACGTATGA