Detailed information of TA system TA101320

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54633..54897
• Replicon: plasmid p2
• Accession: NZ_CP028485
• Organism: Escherichia coli strain E41-1

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: C2U19_RS27395
• Protein ID: WP_001387489.1
• Coordinates: 54745..54897 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	54633..54693 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U19_RS27380	50735..51805	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
C2U19_RS27385	51824..53032	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
C2U19_RS27390	53339..54430	-	1092	WP_000426061.1	hypothetical protein	-
-	54633..54693	-	61	NuclAT_0	-	Antitoxin
-	54633..54693	-	61	NuclAT_0	-	Antitoxin
-	54633..54693	-	61	NuclAT_0	-	Antitoxin
-	54633..54693	-	61	NuclAT_0	-	Antitoxin
C2U19_RS27395	54745..54897	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
C2U19_RS27400	54969..55220	-	252	WP_001291964.1	hypothetical protein	-
C2U19_RS27405	55520..55816	+	297	WP_011264046.1	hypothetical protein	-
C2U19_RS28345	55881..56057	-	177	WP_001054897.1	hypothetical protein	-
C2U19_RS27410	56449..56658	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
C2U19_RS27415	56730..57392	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
C2U19_RS27420	57463..59631	-	2169	WP_058649949.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..86657	86657

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T101320 WP_001387489.1 NZ_CP028485:54745-54897 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T101320 NZ_CP028485:54745-54897 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT101320 NZ_CP028485:c54693-54633 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin