Detailed information of TA system TA101310

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2834..3073
• Replicon: plasmid p1
• Accession: NZ_CP028484
• Organism: Escherichia coli strain E41-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: C2U19_RS26090
• Protein ID: WP_023144756.1
• Coordinates: 2939..3073 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	2834..2894 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U19_RS26070	87..512	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
C2U19_RS26075	509..859	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
C2U19_RS26080	890..2503	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
C2U19_RS26085	2581..2867	+	287	Protein_3	DUF2726 domain-containing protein	-
-	2834..2894	-	61	NuclAT_2	-	Antitoxin
-	2834..2894	-	61	NuclAT_2	-	Antitoxin
-	2834..2894	-	61	NuclAT_2	-	Antitoxin
-	2834..2894	-	61	NuclAT_2	-	Antitoxin
C2U19_RS26090	2939..3073	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
C2U19_RS26095	3370..3624	+	255	WP_000083850.1	replication regulatory protein RepA	-
C2U19_RS28330	3730..3864	+	135	Protein_6	protein CopA/IncA	-
C2U19_RS26105	3861..3935	+	75	WP_031943482.1	RepA leader peptide Tap	-
C2U19_RS26110	3928..4785	+	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
C2U19_RS26120	5725..6378	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
C2U19_RS28140	6471..6728	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
C2U19_RS26125	6661..7062	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	senB	1..128911	128911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T101310 WP_023144756.1 NZ_CP028484:2939-3073 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T101310 NZ_CP028484:2939-3073 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT101310 NZ_CP028484:c2894-2834 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin