Detailed information of TA system TA101254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2427015..2427532
• Replicon: chromosome
• Accession: NZ_CP028470
• Organism: Staphylococcus aureus strain IT4-R

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: C5Y81_RS12510
• Protein ID: WP_000113261.1
• Coordinates: 2427015..2427281 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: C5Y81_RS12515
• Protein ID: WP_000584499.1
• Coordinates: 2427281..2427532 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5Y81_RS12485	2422452..2422988	-	537	WP_000169319.1	GNAT family N-acetyltransferase	-
C5Y81_RS12490	2423222..2424046	-	825	WP_000594640.1	formate/nitrite transporter family protein	-
C5Y81_RS12495	2424249..2424431	-	183	WP_000250735.1	hypothetical protein	-
C5Y81_RS12500	2424518..2424985	-	468	WP_000153345.1	SRPBCC domain-containing protein	-
C5Y81_RS12505	2425171..2426721	-	1551	WP_000727760.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
C5Y81_RS12510	2427015..2427281	-	267	WP_000113261.1	Txe/YoeB family addiction module toxin	Toxin
C5Y81_RS12515	2427281..2427532	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C5Y81_RS12520	2427694..2427795	-	102	Protein_2323	hypothetical protein	-
C5Y81_RS12525	2427802..2428401	-	600	WP_000162810.1	DsbA family protein	-
C5Y81_RS12530	2428420..2428782	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
C5Y81_RS12535	2429037..2430287	+	1251	WP_001012231.1	FemA/FemB family glycyltransferase FmhA	-
C5Y81_RS12540	2430372..2431103	-	732	WP_000615455.1	amino acid ABC transporter ATP-binding protein	-
C5Y81_RS12545	2431100..2431819	-	720	WP_000479573.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10452.02 Da
• Isoelectric Point: 10.0373

>T101254 WP_000113261.1 NZ_CP028470:c2427281-2427015 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDKHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T101254 NZ_CP028470:c2427281-2427015 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAAGCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT101254 WP_000584499.1 NZ_CP028470:c2427532-2427281 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT101254 NZ_CP028470:c2427532-2427281 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAGCACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: T1YB60