Detailed information of TA system TA101214

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27364..27634
• Replicon: plasmid pRM9975-2
• Accession: NZ_CP028434
• Organism: Escherichia coli strain RM9975

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECRM9975_RS28675
• Protein ID: WP_001312861.1
• Coordinates: 27476..27634 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27364..27427 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM9975_RS28630	22425..22685	+	261	WP_071600428.1	hypothetical protein	-
ECRM9975_RS28650	23158..23685	+	528	WP_000290792.1	single-stranded DNA-binding protein	-
ECRM9975_RS28655	23741..23974	+	234	WP_000006004.1	DUF905 domain-containing protein	-
ECRM9975_RS28660	24033..25991	+	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
ECRM9975_RS28665	26046..26480	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
ECRM9975_RS28670	26477..27196	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
ECRM9975_RS29360	27208..27396	-	189	WP_001299721.1	hypothetical protein	-
-	27208..27432	+	225	NuclAT_0	-	-
-	27208..27432	+	225	NuclAT_0	-	-
-	27208..27432	+	225	NuclAT_0	-	-
-	27208..27432	+	225	NuclAT_0	-	-
-	27364..27427	-	64	-	-	Antitoxin
ECRM9975_RS28675	27476..27634	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
ECRM9975_RS28695	28556..28843	+	288	WP_000107535.1	hypothetical protein	-
ECRM9975_RS28700	28964..29785	+	822	WP_001234469.1	DUF945 domain-containing protein	-
ECRM9975_RS28705	30082..30591	-	510	Protein_35	transglycosylase SLT domain-containing protein	-
ECRM9975_RS28710	31005..31388	+	384	WP_001151524.1	relaxosome protein TraM	-
ECRM9975_RS28715	31575..32264	+	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyD / hlyB / hlyA / hlyC	1..78526	78526

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T101214 WP_001312861.1 NZ_CP028434:27476-27634 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T101214 NZ_CP028434:27476-27634 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT101214 NZ_CP028434:c27427-27364 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin