Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2513549..2514051 Replicon chromosome
Accession NZ_CP028432
Organism Escherichia coli strain RM9975

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECRM9975_RS13740 Protein ID WP_000767829.1
Coordinates 2513549..2513803 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECRM9975_RS13745 Protein ID WP_001259255.1
Coordinates 2513800..2514051 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM9975_RS13710 2508564..2508791 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECRM9975_RS13715 2508805..2509863 - 1059 WP_000492340.1 transport protein YeeE -
ECRM9975_RS13720 2510042..2511400 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECRM9975_RS13725 2511390..2511452 - 63 WP_010723108.1 membrane protein YoeI -
ECRM9975_RS13730 2511667..2512596 - 930 WP_000803331.1 LysR family transcriptional regulator -
ECRM9975_RS13735 2512642..2513466 - 825 WP_000754755.1 SDR family oxidoreductase -
ECRM9975_RS13740 2513549..2513803 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECRM9975_RS13745 2513800..2514051 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECRM9975_RS13750 2514335..2514385 + 51 WP_001364200.1 his operon leader peptide -
ECRM9975_RS13760 2514531..2515430 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECRM9975_RS13765 2515436..2516740 + 1305 WP_001345258.1 histidinol dehydrogenase -
ECRM9975_RS13770 2516737..2517816 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
ECRM9975_RS13775 2517813..2518880 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T101203 WP_000767829.1 NZ_CP028432:c2513803-2513549 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T101203 NZ_CP028432:c2513803-2513549 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT101203 WP_001259255.1 NZ_CP028432:c2514051-2513800 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT101203 NZ_CP028432:c2514051-2513800 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References