Detailed information of TA system TA101189

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1652163..1652534
• Replicon: chromosome
• Accession: NZ_CP028432
• Organism: Escherichia coli strain RM9975

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: ECRM9975_RS08830
• Protein ID: WP_001443846.1
• Coordinates: 1652385..1652534 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1652163..1652341 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM9975_RS08800	1647915..1648088	+	174	WP_001296046.1	hypothetical protein	-
ECRM9975_RS08805	1648118..1649491	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
ECRM9975_RS08810	1649620..1650555	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
ECRM9975_RS08815	1650607..1651842	-	1236	WP_000040839.1	site-specific integrase	-
ECRM9975_RS08820	1651844..1652059	-	216	WP_000079604.1	excisionase XisR	-
ECRM9975_RS08825	1652159..1652347	-	189	WP_001302840.1	DUF1187 family protein	-
-	1652163..1652341	+	179	NuclAT_0	-	Antitoxin
-	1652163..1652341	+	179	NuclAT_0	-	Antitoxin
-	1652163..1652341	+	179	NuclAT_0	-	Antitoxin
-	1652163..1652341	+	179	NuclAT_0	-	Antitoxin
ECRM9975_RS08830	1652385..1652534	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ECRM9975_RS08835	1652590..1653399	-	810	WP_000166313.1	recombination protein RecT	-
ECRM9975_RS08840	1653392..1655992	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
ECRM9975_RS08845	1656094..1656369	-	276	WP_001344816.1	hypothetical protein	-
ECRM9975_RS08850	1656444..1656614	-	171	WP_001352098.1	conserved protein, Rac prophage	-
ECRM9975_RS08855	1656614..1656835	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleH2 / nleF / espM1 / paa	1648118..1736013	87895

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T101189 WP_001443846.1 NZ_CP028432:c1652534-1652385 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T101189 NZ_CP028432:c1652534-1652385 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT101189 NZ_CP028432:1652163-1652341 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin