Detailed information of TA system TA101181

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25537..25806
• Replicon: plasmid pRM9131-2
• Accession: NZ_CP028431
• Organism: Escherichia coli strain RM9131

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECRM9131_RS28670
• Protein ID: WP_001312861.1
• Coordinates: 25648..25806 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	25537..25602 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM9131_RS28625	20597..20857	+	261	WP_071600428.1	hypothetical protein	-
ECRM9131_RS28645	21330..21857	+	528	WP_000290792.1	single-stranded DNA-binding protein	-
ECRM9131_RS28650	21913..22146	+	234	WP_000006004.1	DUF905 domain-containing protein	-
ECRM9131_RS28655	22205..24163	+	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
ECRM9131_RS28660	24218..24652	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
ECRM9131_RS28665	24649..25368	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
ECRM9131_RS29350	25380..25568	-	189	WP_001299721.1	hypothetical protein	-
-	25380..25604	+	225	NuclAT_0	-	-
-	25380..25604	+	225	NuclAT_0	-	-
-	25380..25604	+	225	NuclAT_0	-	-
-	25380..25604	+	225	NuclAT_0	-	-
-	25537..25602	+	66	-	-	Antitoxin
ECRM9131_RS28670	25648..25806	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
ECRM9131_RS28690	26728..27015	+	288	WP_000107535.1	hypothetical protein	-
ECRM9131_RS28695	27136..27957	+	822	WP_001234469.1	DUF945 domain-containing protein	-
ECRM9131_RS28700	28254..28763	-	510	Protein_33	transglycosylase SLT domain-containing protein	-
ECRM9131_RS28705	29177..29560	+	384	WP_001151524.1	relaxosome protein TraM	-
ECRM9131_RS28710	29747..30436	+	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyC / hlyA / hlyB / hlyD	1..76698	76698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T101181 WP_001312861.1 NZ_CP028431:25648-25806 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T101181 NZ_CP028431:25648-25806 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT101181 NZ_CP028431:25537-25602 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin