Detailed information of TA system TA101174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3815948..3816747
• Replicon: chromosome
• Accession: NZ_CP028429
• Organism: Escherichia coli strain RM9131

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: ECRM9131_RS20450
• Protein ID: WP_000347273.1
• Coordinates: 3816283..3816747 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: ECRM9131_RS20445
• Protein ID: WP_001307405.1
• Coordinates: 3815948..3816283 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM9131_RS20430	3811733..3812503	-	771	WP_001058225.1	2-dehydro-3-deoxyglucarate aldolase	-
ECRM9131_RS20435	3812519..3813853	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
ECRM9131_RS20440	3814228..3815799	+	1572	WP_001273741.1	galactarate dehydratase	-
ECRM9131_RS20445	3815948..3816283	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ECRM9131_RS20450	3816283..3816747	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ECRM9131_RS20455	3816802..3817611	-	810	WP_000072193.1	aga operon transcriptional regulator AgaR	-
ECRM9131_RS20465	3817860..3819140	+	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ECRM9131_RS20470	3819163..3819636	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ECRM9131_RS20475	3819647..3820426	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ECRM9131_RS20480	3820416..3821294	+	879	WP_001341890.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ECRM9131_RS20485	3821312..3821746	+	435	WP_000948822.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3805292..3816747	11455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T101174 WP_000347273.1 NZ_CP028429:3816283-3816747 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T101174 NZ_CP028429:3816283-3816747 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT101174 WP_001307405.1 NZ_CP028429:3815948-3816283 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT101174 NZ_CP028429:3815948-3816283 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
ACCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7