Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2518856..2519358 Replicon chromosome
Accession NZ_CP028429
Organism Escherichia coli strain RM9131

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECRM9131_RS13765 Protein ID WP_000767829.1
Coordinates 2518856..2519110 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECRM9131_RS13770 Protein ID WP_001259255.1
Coordinates 2519107..2519358 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM9131_RS13735 2513871..2514098 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECRM9131_RS13740 2514112..2515170 - 1059 WP_000492340.1 transport protein YeeE -
ECRM9131_RS13745 2515349..2516707 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECRM9131_RS13750 2516697..2516759 - 63 WP_010723108.1 membrane protein YoeI -
ECRM9131_RS13755 2516974..2517903 - 930 WP_000803331.1 LysR family transcriptional regulator -
ECRM9131_RS13760 2517949..2518773 - 825 WP_000754755.1 SDR family oxidoreductase -
ECRM9131_RS13765 2518856..2519110 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECRM9131_RS13770 2519107..2519358 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECRM9131_RS13775 2519642..2519692 + 51 WP_001364200.1 his operon leader peptide -
ECRM9131_RS13785 2519838..2520737 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECRM9131_RS13790 2520743..2522047 + 1305 WP_001345258.1 histidinol dehydrogenase -
ECRM9131_RS13795 2522044..2523123 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
ECRM9131_RS13800 2523120..2524187 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T101169 WP_000767829.1 NZ_CP028429:c2519110-2518856 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T101169 NZ_CP028429:c2519110-2518856 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT101169 WP_001259255.1 NZ_CP028429:c2519358-2519107 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT101169 NZ_CP028429:c2519358-2519107 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References