Detailed information of TA system TA101155

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1656156..1656527
• Replicon: chromosome
• Accession: NZ_CP028429
• Organism: Escherichia coli strain RM9131

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: ECRM9131_RS08845
• Protein ID: WP_001443846.1
• Coordinates: 1656378..1656527 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1656156..1656334 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM9131_RS08815	1651908..1652081	+	174	WP_001296046.1	hypothetical protein	-
ECRM9131_RS08820	1652111..1653484	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
ECRM9131_RS08825	1653613..1654548	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
ECRM9131_RS08830	1654600..1655835	-	1236	WP_000040839.1	site-specific integrase	-
ECRM9131_RS08835	1655837..1656052	-	216	WP_000079604.1	excisionase XisR	-
ECRM9131_RS08840	1656152..1656340	-	189	WP_001302840.1	DUF1187 family protein	-
-	1656156..1656334	+	179	NuclAT_0	-	Antitoxin
-	1656156..1656334	+	179	NuclAT_0	-	Antitoxin
-	1656156..1656334	+	179	NuclAT_0	-	Antitoxin
-	1656156..1656334	+	179	NuclAT_0	-	Antitoxin
ECRM9131_RS08845	1656378..1656527	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ECRM9131_RS08850	1656583..1657392	-	810	WP_000166313.1	recombination protein RecT	-
ECRM9131_RS08855	1657385..1659985	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
ECRM9131_RS08860	1660087..1660362	-	276	WP_001344816.1	hypothetical protein	-
ECRM9131_RS08865	1660437..1660607	-	171	WP_001352098.1	conserved protein, Rac prophage	-
ECRM9131_RS08870	1660607..1660828	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleH2 / nleF / espM1 / paa / nleG7'	1654600..1756931	102331

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T101155 WP_001443846.1 NZ_CP028429:c1656527-1656378 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T101155 NZ_CP028429:c1656527-1656378 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT101155 NZ_CP028429:1656156-1656334 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin