Detailed information of TA system TA101145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 1434349..1434997
• Replicon: chromosome
• Accession: NZ_CP028414
• Organism: Streptococcus mitis NCTC 12261

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V8IC77
• Locus tag: SM12261_RS07260
• Protein ID: WP_000556942.1
• Coordinates: 1434632..1434997 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V8I9N0
• Locus tag: SM12261_RS07255
• Protein ID: WP_000797746.1
• Coordinates: 1434349..1434642 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM12261_RS07245	1430605..1431471	-	867	WP_000089956.1	methylenetetrahydrofolate reductase [NAD(P)H]	-
SM12261_RS07250	1431575..1433824	-	2250	WP_000108228.1	5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase	-
SM12261_RS07255	1434349..1434642	-	294	WP_000797746.1	helix-turn-helix transcriptional regulator	Antitoxin
SM12261_RS07260	1434632..1434997	-	366	WP_000556942.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SM12261_RS07265	1435153..1435518	-	366	WP_001241814.1	TIGR02328 family protein	-
SM12261_RS07270	1435502..1435906	-	405	WP_000527884.1	YbgA family protein	-
SM12261_RS07275	1435974..1436675	-	702	WP_000748126.1	hypothetical protein	-
SM12261_RS07280	1436810..1438198	-	1389	WP_115262365.1	hypothetical protein	-
SM12261_RS07285	1438195..1439262	-	1068	WP_000415506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-88)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14545.72 Da
• Isoelectric Point: 9.3143

>T101145 WP_000556942.1 NZ_CP028414:c1434997-1434632 [Streptococcus mitis NCTC 12261]
VHTIYFYKDKNGNEPVLDYMRELACRKSKDSRIKLNKLNDYIELLSQQGTRAGEPYMKHLEDEIWELRPLRDRILFVAWL
DGSFVLLHHFVKKTQKTPRREIEKAKRELKDLKERGVSDEE

Nucleotide

• Length: 366 bp

>T101145 NZ_CP028414:c1434997-1434632 [Streptococcus mitis NCTC 12261]
GTGCATACGATTTACTTCTATAAGGACAAAAATGGAAATGAGCCAGTCTTGGATTATATGAGAGAATTGGCCTGTCGTAA
AAGTAAGGATAGCCGCATCAAACTCAATAAACTAAATGACTATATCGAGTTACTTAGTCAGCAAGGGACCAGAGCTGGGG
AACCTTATATGAAACACTTGGAAGATGAGATATGGGAACTAAGACCTTTGAGAGATCGAATTTTATTTGTAGCGTGGCTT
GATGGTAGTTTTGTTCTTTTACATCATTTTGTCAAAAAGACTCAGAAAACTCCTAGAAGAGAAATTGAAAAAGCCAAGCG
TGAACTGAAGGACTTAAAAGAAAGAGGGGTAAGTGATGAAGAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10765.46 Da
• Isoelectric Point: 7.0172

>AT101145 WP_000797746.1 NZ_CP028414:c1434642-1434349 [Streptococcus mitis NCTC 12261]
MKNSAIGSNWKDVRSELFTKEEILESDMRVAIMSELIEARHEQGISQKKLEELSGVSQPVIARMETGKTSPQLDTVLKVL
ASLGKTLAVVPLEQRKS

Nucleotide

• Length: 294 bp

>AT101145 NZ_CP028414:c1434642-1434349 [Streptococcus mitis NCTC 12261]
ATGAAGAATAGTGCTATTGGGAGTAACTGGAAGGATGTCAGGTCAGAACTCTTTACCAAGGAGGAAATACTTGAAAGTGA
TATGCGAGTGGCTATCATGAGTGAGTTGATTGAAGCCAGACATGAGCAAGGAATCAGCCAGAAAAAGCTAGAGGAACTCA
GTGGAGTGAGCCAGCCTGTCATAGCTAGGATGGAAACAGGAAAGACAAGTCCTCAGTTGGATACAGTCTTGAAAGTTTTA
GCCAGTTTAGGAAAGACACTAGCAGTCGTCCCACTTGAACAGAGAAAGAGTTGA

Structures

Toxin

• AlphaFold DB: V8IC77

Antitoxin

• AlphaFold DB: V8I9N0