Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 168523..169247 Replicon plasmid unnamed
Accession NZ_CP028385
Organism Providencia heimbachae strain 99101

Toxin (Protein)


Gene name higB Uniprot ID A0A4D7JCU9
Locus tag C9446_RS20835 Protein ID WP_067423691.1
Coordinates 168936..169247 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4D7J260
Locus tag C9446_RS20830 Protein ID WP_067423690.1
Coordinates 168523..168939 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9446_RS20785 163653..164075 + 423 WP_137022573.1 hypothetical protein -
C9446_RS20790 164244..164801 + 558 WP_137022574.1 hypothetical protein -
C9446_RS20795 164940..165293 + 354 WP_137022575.1 hypothetical protein -
C9446_RS20800 165290..165508 + 219 WP_137022576.1 hypothetical protein -
C9446_RS20805 165845..166342 + 498 WP_137022577.1 hypothetical protein -
C9446_RS20810 166443..166826 + 384 WP_137022578.1 hypothetical protein -
C9446_RS20815 166890..167666 + 777 WP_067421877.1 methyltransferase -
C9446_RS20820 167798..168133 + 336 WP_137022579.1 hypothetical protein -
C9446_RS20825 168159..168464 + 306 WP_137022580.1 hypothetical protein -
C9446_RS20830 168523..168939 - 417 WP_067423690.1 helix-turn-helix domain-containing protein Antitoxin
C9446_RS20835 168936..169247 - 312 WP_067423691.1 type II toxin-antitoxin system HigB family toxin Toxin
C9446_RS20840 169418..169984 + 567 WP_137022581.1 hypothetical protein -
C9446_RS20845 170107..170424 + 318 WP_067423694.1 hypothetical protein -
C9446_RS20850 170421..170726 + 306 WP_067423696.1 hypothetical protein -
C9446_RS20855 171029..171904 + 876 WP_137022582.1 repA protein -
C9446_RS20860 172445..172930 + 486 WP_067423698.1 hypothetical protein -
C9446_RS20865 172993..173442 + 450 WP_137022583.1 hypothetical protein -
C9446_RS20870 173613..174095 + 483 WP_137022584.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catB8 / blaOXA-10 / ant(3'')-Ia / dfrA1 / qacE / sul1 - 1..231957 231957


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(82-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12375.16 Da        Isoelectric Point: 10.3654

>T101119 WP_067423691.1 NZ_CP028385:c169247-168936 [Providencia heimbachae]
MHVISRDPFNQASKRFPNSAQALSDVYRTLKRDSYQTPDELKKVFPSLDRMKYREKWWVIDISGNALRMMFFADFDRGKI
FVKHITTHAEYDKLTDHYRRTKA

Download         Length: 312 bp

>T101119 NZ_CP028385:c169247-168936 [Providencia heimbachae]
ATGCACGTAATTTCAAGAGATCCTTTTAACCAGGCATCCAAGCGATTTCCTAACAGTGCTCAGGCGTTATCTGACGTATA
TCGAACACTTAAGCGCGATAGTTATCAGACACCCGATGAGCTTAAGAAGGTTTTCCCCAGCCTTGACCGAATGAAGTACA
GAGAGAAATGGTGGGTTATCGATATCAGTGGAAATGCGTTAAGAATGATGTTTTTCGCCGACTTTGACCGGGGAAAAATA
TTCGTGAAACACATAACGACCCACGCAGAGTACGATAAGTTGACAGATCACTACCGGAGAACAAAAGCATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15451.44 Da        Isoelectric Point: 4.6155

>AT101119 WP_067423690.1 NZ_CP028385:c168939-168523 [Providencia heimbachae]
MMFQDAVKAAQNLVNLVPLLGDSHSRPDYEKAVQLVEHLVENDPDNPLIDMICAKIDAYENAAPEFAEFNQRLAESNDGV
AALRTLMDQYNLNTTDFGNELGSRSYVSRILNGERGLTLEHIKNLSKRFGIPASIFIR

Download         Length: 417 bp

>AT101119 NZ_CP028385:c168939-168523 [Providencia heimbachae]
ATGATGTTTCAAGACGCCGTAAAAGCGGCACAAAACCTGGTAAATCTAGTCCCGTTACTGGGAGACAGCCATTCACGCCC
GGATTATGAAAAGGCCGTTCAGTTGGTGGAGCATCTTGTCGAGAACGACCCTGACAATCCGCTCATCGATATGATTTGCG
CAAAAATAGATGCGTATGAAAATGCCGCACCTGAATTTGCAGAGTTTAACCAACGTTTAGCAGAAAGTAATGATGGTGTG
GCAGCGTTAAGAACGCTGATGGATCAATACAACCTCAATACGACAGACTTTGGAAATGAGCTCGGTTCTCGTTCATACGT
CAGCCGTATCCTCAATGGGGAACGTGGATTAACCTTGGAGCACATCAAAAATCTGTCCAAACGCTTTGGTATTCCAGCCT
CAATATTTATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D7JCU9

Antitoxin

Source ID Structure
AlphaFold DB A0A4D7J260

References