Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 714178..714748 Replicon chromosome
Accession NZ_CP028384
Organism Providencia heimbachae strain 99101

Toxin (Protein)


Gene name higB Uniprot ID A0A4D7IT23
Locus tag C9446_RS03060 Protein ID WP_068439147.1
Coordinates 714178..714459 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A1B7JQM8
Locus tag C9446_RS03065 Protein ID WP_068439151.1
Coordinates 714470..714748 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9446_RS03045 709412..710644 + 1233 WP_068439144.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
C9446_RS03050 710719..712385 - 1667 Protein_596 energy-dependent translational throttle protein EttA -
C9446_RS03055 712900..713931 - 1032 WP_137021093.1 IS630 family transposase -
C9446_RS03060 714178..714459 + 282 WP_068439147.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C9446_RS03065 714470..714748 + 279 WP_068439151.1 HigA family addiction module antidote protein Antitoxin
C9446_RS03070 714958..716874 + 1917 WP_175403093.1 murein transglycosylase -
C9446_RS03075 717054..717371 + 318 WP_068439659.1 trp operon repressor -
C9446_RS03080 717457..717993 - 537 WP_068439155.1 inosine/xanthosine triphosphatase -
C9446_RS03085 718046..718690 + 645 WP_068439157.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase GpmB -
C9446_RS03090 718737..719687 - 951 WP_137021215.1 MDR efflux pump AcrAB transcriptional activator RobA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 712900..713931 1031


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-64)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10733.39 Da        Isoelectric Point: 10.6271

>T101107 WP_068439147.1 NZ_CP028384:714178-714459 [Providencia heimbachae]
VIKSFKHKGIERFFKAGITSGIQVKHITKLRIQLTALNTAKKPNDMSAPSWKLHQLKGADLQNHWSISVNGNWRLTFKFE
GENVILVDYQDYH

Download         Length: 282 bp

>T101107 NZ_CP028384:714178-714459 [Providencia heimbachae]
GTGATAAAAAGTTTTAAGCACAAAGGAATAGAGCGGTTTTTTAAAGCGGGAATAACATCGGGTATTCAAGTAAAGCATAT
AACTAAACTACGGATACAGTTAACGGCGCTGAATACGGCTAAAAAACCTAACGACATGTCTGCGCCGAGTTGGAAGTTAC
ATCAATTAAAGGGTGCTGATTTACAAAACCATTGGTCAATATCCGTAAATGGAAATTGGCGGTTAACGTTTAAATTTGAG
GGTGAGAATGTCATTCTCGTCGACTACCAAGATTATCATTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 9991.52 Da        Isoelectric Point: 7.3174

>AT101107 WP_068439151.1 NZ_CP028384:714470-714748 [Providencia heimbachae]
MSKMHNPEHPGLVLREYLDGISVTDAAKALGVTRATLSRILNGNSGISADMALRLEIALGTSAEMWVNMQAQYELWQAAQ
GTRPVVKAIFAH

Download         Length: 279 bp

>AT101107 NZ_CP028384:714470-714748 [Providencia heimbachae]
ATGAGCAAAATGCATAATCCAGAGCATCCAGGTTTAGTATTACGCGAATATTTAGATGGTATTTCTGTCACTGACGCAGC
AAAAGCATTGGGTGTGACTCGAGCTACTTTATCTCGTATTTTGAATGGAAATAGTGGAATTTCAGCGGATATGGCATTAA
GGCTTGAAATTGCACTAGGGACGAGTGCAGAAATGTGGGTTAATATGCAAGCCCAATATGAATTATGGCAAGCAGCTCAA
GGTACAAGGCCTGTCGTAAAGGCAATCTTTGCGCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D7IT23

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JQM8

References