Detailed information of TA system TA101106

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42491..42908
• Replicon: plasmid pRM10466-2
• Accession: NZ_CP028383
• Organism: Escherichia coli strain RM10466

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: C7R96_RS27710
• Protein ID: WP_000775238.1
• Coordinates: 42747..42908 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	42491..42682 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7R96_RS27675	38172..38693	+	522	WP_000290783.1	single-stranded DNA-binding protein	-
C7R96_RS27685	38751..38984	+	234	WP_069192978.1	DUF905 domain-containing protein	-
C7R96_RS27690	39045..41009	+	1965	WP_087672663.1	ParB/RepB/Spo0J family partition protein	-
C7R96_RS27695	41078..41512	+	435	WP_000845923.1	conjugation system SOS inhibitor PsiB	-
C7R96_RS27700	41509..42228	+	720	WP_001276237.1	plasmid SOS inhibition protein A	-
C7R96_RS27705	42225..42539	+	315	WP_000872084.1	hypothetical protein	-
-	42488..42682	+	195	NuclAT_0	-	-
-	42488..42682	+	195	NuclAT_0	-	-
-	42488..42682	+	195	NuclAT_0	-	-
-	42488..42682	+	195	NuclAT_0	-	-
-	42491..42682	-	192	-	-	Antitoxin
C7R96_RS27710	42747..42908	+	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
C7R96_RS28435	43209..43506	-	298	Protein_49	hypothetical protein	-
C7R96_RS27730	43803..44105	+	303	WP_001272235.1	hypothetical protein	-
C7R96_RS27740	44261..44548	+	288	WP_000107543.1	hypothetical protein	-
C7R96_RS27745	44667..45488	+	822	WP_032107531.1	DUF945 domain-containing protein	-
C7R96_RS27750	45785..46387	-	603	WP_077785958.1	transglycosylase SLT domain-containing protein	-
C7R96_RS27755	46727..47110	+	384	WP_032210979.1	relaxosome protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67750	67750

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T101106 WP_000775238.1 NZ_CP028383:42747-42908 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T101106 NZ_CP028383:42747-42908 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 192 bp

>AT101106 NZ_CP028383:c42682-42491 [Escherichia coli]
GCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACAACACGGTAACAAACCACCTTCACGTC
ATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCGCACTGACCACACTCACTTTCCCTGAAAAT
AATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin