Detailed information of TA system TA101098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4495753..4496355
• Replicon: chromosome
• Accession: NZ_CP028381
• Organism: Escherichia coli strain RM10466

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C7R96_RS23700
• Protein ID: WP_000897305.1
• Coordinates: 4495753..4496064 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7R96_RS23705
• Protein ID: WP_000356397.1
• Coordinates: 4496065..4496355 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7R96_RS23675	4491667..4492266	+	600	WP_001295269.1	glucose-1-phosphatase	-
C7R96_RS23680	4492260..4493132	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C7R96_RS23685	4493129..4493566	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C7R96_RS23690	4493611..4494552	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C7R96_RS23695	4494616..4495524	-	909	WP_001162704.1	alpha/beta hydrolase	-
C7R96_RS23700	4495753..4496064	+	312	WP_000897305.1	hypothetical protein	Toxin
C7R96_RS23705	4496065..4496355	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C7R96_RS23715	4496960..4497178	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C7R96_RS23720	4497397..4497639	+	243	WP_001087409.1	hypothetical protein	-
C7R96_RS23725	4497969..4498898	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
C7R96_RS23730	4498895..4499530	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C7R96_RS23735	4499527..4500429	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T101098 WP_000897305.1 NZ_CP028381:4495753-4496064 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T101098 NZ_CP028381:4495753-4496064 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTTGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT101098 WP_000356397.1 NZ_CP028381:4496065-4496355 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT101098 NZ_CP028381:4496065-4496355 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7