Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2375497..2375999 Replicon chromosome
Accession NZ_CP028381
Organism Escherichia coli strain RM10466

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7R96_RS12855 Protein ID WP_000767829.1
Coordinates 2375497..2375751 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C7R96_RS12860 Protein ID WP_001259255.1
Coordinates 2375748..2375999 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7R96_RS12825 2370516..2370743 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C7R96_RS12830 2370757..2371815 - 1059 WP_000492346.1 transport protein YeeE -
C7R96_RS12835 2371990..2373348 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7R96_RS12840 2373338..2373400 - 63 WP_010723108.1 membrane protein YoeI -
C7R96_RS12845 2373615..2374544 - 930 WP_000803351.1 LysR family transcriptional regulator -
C7R96_RS12850 2374590..2375414 - 825 WP_000754749.1 SDR family oxidoreductase -
C7R96_RS12855 2375497..2375751 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7R96_RS12860 2375748..2375999 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7R96_RS12865 2376282..2376332 + 51 WP_001364200.1 his operon leader peptide -
C7R96_RS12875 2376478..2377377 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C7R96_RS12880 2377383..2378687 + 1305 WP_001369878.1 histidinol dehydrogenase -
C7R96_RS12885 2378684..2379754 + 1071 WP_000108962.1 histidinol-phosphate transaminase -
C7R96_RS12890 2379754..2380821 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T101091 WP_000767829.1 NZ_CP028381:c2375751-2375497 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T101091 NZ_CP028381:c2375751-2375497 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT101091 WP_001259255.1 NZ_CP028381:c2375999-2375748 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT101091 NZ_CP028381:c2375999-2375748 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References