Detailed information of TA system TA101070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3756577..3757270
• Replicon: chromosome
• Accession: NZ_CP028379
• Organism: Escherichia coli O145 str. RM9872

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: I3Y_RS19450
• Protein ID: WP_000415584.1
• Coordinates: 3756974..3757270 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: I3Y_RS19445
• Protein ID: WP_000650107.1
• Coordinates: 3756577..3756972 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3Y_RS19435	3752441..3754699	-	2259	WP_001281878.1	DNA topoisomerase IV subunit A	-
I3Y_RS19440	3754837..3756444	-	1608	WP_001400746.1	ABC transporter substrate-binding protein	-
I3Y_RS19445	3756577..3756972	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
I3Y_RS19450	3756974..3757270	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
I3Y_RS19455	3757475..3757957	-	483	WP_000183497.1	GyrI-like domain-containing protein	-
I3Y_RS19460	3758010..3758402	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
I3Y_RS19465	3758554..3759213	+	660	WP_001221502.1	two-component system response regulator QseB	-
I3Y_RS19470	3759210..3760559	+	1350	WP_000673396.1	two-component system sensor histidine kinase QseC	-
I3Y_RS19475	3760641..3760937	-	297	WP_000912118.1	DUF2645 family protein	-
I3Y_RS19480	3761295..3761876	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
I3Y_RS19485	3761907..3762221	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T101070 WP_000415584.1 NZ_CP028379:c3757270-3756974 [Escherichia coli O145 str. RM9872]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T101070 NZ_CP028379:c3757270-3756974 [Escherichia coli O145 str. RM9872]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGACTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT101070 WP_000650107.1 NZ_CP028379:c3756972-3756577 [Escherichia coli O145 str. RM9872]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT101070 NZ_CP028379:c3756972-3756577 [Escherichia coli O145 str. RM9872]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACACAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65