Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2633124..2633626 Replicon chromosome
Accession NZ_CP028379
Organism Escherichia coli O145 str. RM9872

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag I3Y_RS14030 Protein ID WP_000767829.1
Coordinates 2633124..2633378 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I3Y_RS14035 Protein ID WP_001259255.1
Coordinates 2633375..2633626 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3Y_RS14000 2628139..2628366 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
I3Y_RS14005 2628380..2629438 - 1059 WP_000492332.1 transport protein YeeE -
I3Y_RS14010 2629617..2630975 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I3Y_RS14015 2630965..2631027 - 63 WP_010723108.1 membrane protein YoeI -
I3Y_RS14020 2631242..2632171 - 930 WP_000803351.1 LysR family transcriptional regulator -
I3Y_RS14025 2632217..2633041 - 825 WP_000754755.1 SDR family oxidoreductase -
I3Y_RS14030 2633124..2633378 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I3Y_RS14035 2633375..2633626 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I3Y_RS14040 2633910..2633960 + 51 WP_001364200.1 his operon leader peptide -
I3Y_RS14050 2634106..2635005 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
I3Y_RS14055 2635011..2636315 + 1305 WP_001400693.1 histidinol dehydrogenase -
I3Y_RS14060 2636312..2637382 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
I3Y_RS14065 2637382..2638449 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T101066 WP_000767829.1 NZ_CP028379:c2633378-2633124 [Escherichia coli O145 str. RM9872]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T101066 NZ_CP028379:c2633378-2633124 [Escherichia coli O145 str. RM9872]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT101066 WP_001259255.1 NZ_CP028379:c2633626-2633375 [Escherichia coli O145 str. RM9872]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT101066 NZ_CP028379:c2633626-2633375 [Escherichia coli O145 str. RM9872]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References