Detailed information of TA system TA101053

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1725109..1725480
• Replicon: chromosome
• Accession: NZ_CP028379
• Organism: Escherichia coli O145 str. RM9872

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: I3Y_RS08855
• Protein ID: WP_021500490.1
• Coordinates: 1725286..1725480 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1725109..1725287 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3Y_RS08830	1721063..1722436	+	1374	WP_000123735.1	ATP-dependent RNA helicase DbpA	-
I3Y_RS08835	1722565..1723500	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
I3Y_RS08840	1723553..1724788	-	1236	WP_000040838.1	site-specific integrase	-
I3Y_RS08845	1724790..1725005	-	216	WP_000079604.1	excisionase XisR	-
I3Y_RS08850	1725105..1725293	-	189	WP_001298826.1	DUF1187 family protein	-
-	1725109..1725287	+	179	NuclAT_0	-	Antitoxin
-	1725109..1725287	+	179	NuclAT_0	-	Antitoxin
-	1725109..1725287	+	179	NuclAT_0	-	Antitoxin
-	1725109..1725287	+	179	NuclAT_0	-	Antitoxin
I3Y_RS08855	1725286..1725480	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
I3Y_RS08860	1725544..1726596	-	1053	WP_001004423.1	recombinase RecT	-
I3Y_RS08865	1726608..1729733	-	3126	WP_000102216.1	exodeoxyribonuclease VIII	-
I3Y_RS08870	1729835..1730110	-	276	WP_001519830.1	phage protein	-
I3Y_RS08875	1730185..1730361	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	1723553..1789917	66364

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T101053 WP_021500490.1 NZ_CP028379:c1725480-1725286 [Escherichia coli O145 str. RM9872]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T101053 NZ_CP028379:c1725480-1725286 [Escherichia coli O145 str. RM9872]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT101053 NZ_CP028379:1725109-1725287 [Escherichia coli O145 str. RM9872]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin