Detailed information of TA system TA101025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 215841..216559
• Replicon: plasmid pPV989-508
• Accession: NZ_CP028350
• Organism: Pantoea vagans strain PV989

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C9381_RS20200
• Protein ID: WP_107320313.1
• Coordinates: 215841..216152 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C9381_RS20205
• Protein ID: WP_107320314.1
• Coordinates: 216149..216559 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9381_RS20180	211130..212236	+	1107	WP_107320455.1	site-specific integrase	-
C9381_RS20185	212254..212826	-	573	WP_107320311.1	lipid IV(A) palmitoyltransferase PagP	-
C9381_RS20195	213270..215492	+	2223	WP_107320312.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
C9381_RS20200	215841..216152	+	312	WP_107320313.1	type II toxin-antitoxin system HigB family toxin	Toxin
C9381_RS20205	216149..216559	+	411	WP_107320314.1	helix-turn-helix domain-containing protein	Antitoxin
C9381_RS20210	216675..217520	-	846	WP_107320315.1	VOC family protein	-
C9381_RS20215	217541..218680	-	1140	WP_107320316.1	amidohydrolase	-
C9381_RS20220	218692..219783	-	1092	WP_107320317.1	alkylhydroperoxidase domain protein	-
C9381_RS20225	219780..220787	-	1008	WP_107320318.1	putative FMN-dependent luciferase-like monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN / pilW	1..507680	507680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Antitoxin HigA (5-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12086.88 Da
• Isoelectric Point: 8.8667

>T101025 WP_107320313.1 NZ_CP028350:215841-216152 [Pantoea vagans]
MHVISRVPFDEAARKDPNAAAALDLLYRHLRQGDFSGPDDLRALYASLDRMTYRAKWWVIDVSGNPLRVIFFADFERSKI
FIKHICTHAEYDKLVKKYREVTE

Nucleotide

• Length: 312 bp

>T101025 NZ_CP028350:215841-216152 [Pantoea vagans]
ATGCATGTTATTTCACGCGTGCCGTTTGACGAGGCGGCCAGAAAAGATCCCAATGCAGCGGCAGCACTGGATCTACTTTA
CCGCCACCTCAGACAGGGCGATTTCAGTGGTCCTGACGATCTCAGGGCGCTTTATGCAAGCCTTGATCGTATGACGTATC
GCGCTAAATGGTGGGTCATTGACGTCAGTGGCAACCCTTTGCGCGTGATATTCTTCGCGGATTTTGAGCGCAGCAAAATC
TTCATCAAGCATATCTGCACTCACGCTGAGTACGACAAGCTTGTTAAGAAATATCGGGAGGTAACCGAATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15228.20 Da
• Isoelectric Point: 4.7755

>AT101025 WP_107320314.1 NZ_CP028350:216149-216559 [Pantoea vagans]
MNTEAIRLSNQLAEAVPLLAGSNQRHDYEEALELVEYLIEHEPDNPLVDLLCARIAHYEDNDPAMADFNSRQREISTGPG
VLRVLIAQHNLSLSDFENEIGKKSLVSRILSGDRQLTVSHIRALCARFGLSPSLFF

Nucleotide

• Length: 411 bp

>AT101025 NZ_CP028350:216149-216559 [Pantoea vagans]
ATGAATACTGAAGCTATCAGGCTGTCAAATCAGCTGGCAGAAGCGGTGCCGCTGCTGGCCGGCAGCAACCAGCGACATGA
TTATGAAGAAGCACTGGAACTGGTCGAATACCTTATTGAACATGAGCCGGATAATCCGCTGGTAGATTTGCTTTGTGCCC
GCATTGCTCATTATGAAGATAATGATCCTGCGATGGCCGACTTTAATAGCCGCCAGCGTGAAATCAGCACCGGACCTGGC
GTCCTGAGGGTACTGATCGCGCAGCACAATCTCAGCCTGAGTGACTTTGAAAATGAAATTGGCAAAAAGTCGCTGGTCAG
CCGAATCCTCAGCGGCGATCGTCAGTTGACCGTGTCACACATACGGGCATTGTGCGCGCGGTTTGGCCTGTCACCATCGT
TGTTTTTTTGA