Detailed information of TA system TA101010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 9834..10426
• Replicon: plasmid pVpR13_19Kb
• Accession: NZ_CP028344
• Organism: Vibrio parahaemolyticus isolate R13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C7Y67_RS25915
• Protein ID: WP_080608540.1
• Coordinates: 10109..10426 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7Y67_RS25910
• Protein ID: WP_080608539.1
• Coordinates: 9834..10112 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7Y67_RS25885	6264..6821	+	558	WP_031419876.1	recombinase family protein	-
C7Y67_RS25890	7226..8104	-	879	WP_108745456.1	tyrosine-type recombinase/integrase	-
C7Y67_RS25895	8327..8701	-	375	WP_080608536.1	hypothetical protein	-
C7Y67_RS25900	8698..9213	-	516	WP_080608537.1	ParA family protein	-
C7Y67_RS25905	9420..9794	+	375	WP_108745457.1	DUF4236 domain-containing protein	-
C7Y67_RS25910	9834..10112	-	279	WP_080608539.1	helix-turn-helix transcriptional regulator	Antitoxin
C7Y67_RS25915	10109..10426	-	318	WP_080608540.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7Y67_RS25925	11359..12243	+	885	WP_108745458.1	site-specific integrase	-
C7Y67_RS25930	12240..12899	+	660	WP_108745459.1	hypothetical protein	-
C7Y67_RS25935	12948..13340	+	393	WP_108745460.1	hypothetical protein	-
C7Y67_RS26835	13343..13510	+	168	WP_159087269.1	hypothetical protein	-
C7Y67_RS25940	13570..13857	+	288	WP_080608545.1	hypothetical protein	-
C7Y67_RS25945	13898..14920	-	1023	WP_103199658.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..19217	19217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-86)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12017.19 Da
• Isoelectric Point: 9.8772

>T101010 WP_080608540.1 NZ_CP028344:c10426-10109 [Vibrio parahaemolyticus]
MAWKVDFHDGVEDEILSMPPKIQARMIKLLELIEMHGANLGPPHTEAMGDGLFEIRAKAKEGIGRGLFCYLKGQHVYVLR
AFVKKSNKTPKKEIELARERMREVK

Nucleotide

• Length: 318 bp

>T101010 NZ_CP028344:c10426-10109 [Vibrio parahaemolyticus]
ATGGCTTGGAAAGTAGATTTTCATGATGGTGTTGAGGATGAAATCCTATCAATGCCGCCAAAAATCCAAGCACGTATGAT
TAAGCTATTAGAGCTGATCGAGATGCATGGGGCCAACTTGGGACCGCCGCATACAGAAGCAATGGGTGATGGTCTTTTTG
AGATCAGAGCTAAAGCAAAAGAGGGGATCGGTCGAGGTTTGTTTTGTTATCTAAAGGGGCAACATGTCTATGTTCTTAGG
GCCTTTGTGAAGAAGTCTAACAAAACACCAAAGAAAGAGATCGAACTGGCCCGAGAAAGAATGAGAGAGGTGAAATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10183.72 Da
• Isoelectric Point: 8.0019

>AT101010 WP_080608539.1 NZ_CP028344:c10112-9834 [Vibrio parahaemolyticus]
MMSLAALKKKAFTNQEVKEAYDELGDEFELISTLITMREKSGLTQDEVAQKMGTRAPNISRLESGRGNPSLKTLVSYAQA
CGFKLDLGFKHV

Nucleotide

• Length: 279 bp

>AT101010 NZ_CP028344:c10112-9834 [Vibrio parahaemolyticus]
ATGATGAGCTTAGCTGCATTGAAAAAGAAAGCTTTTACCAATCAAGAGGTGAAAGAAGCGTACGATGAGCTTGGTGATGA
ATTTGAGCTCATTAGTACTCTGATCACGATGAGAGAAAAATCAGGACTGACTCAGGATGAAGTTGCCCAAAAAATGGGAA
CCCGAGCTCCTAATATTTCTCGCCTAGAGTCAGGTAGAGGAAACCCTAGCTTAAAAACCCTCGTTAGCTATGCTCAAGCG
TGTGGCTTCAAGTTAGACCTTGGCTTTAAGCATGTTTGA