Detailed information of TA system TA100993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 991373..991979
• Replicon: chromosome
• Accession: NZ_CP028339
• Organism: Thauera aromatica K172

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: Tharo_RS04775
• Protein ID: WP_107220216.1
• Coordinates: 991373..991696 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Tharo_RS04780
• Protein ID: WP_107220217.1
• Coordinates: 991689..991979 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Tharo_RS04740	986775..987875	+	1101	WP_107220211.1	transposase	-
Tharo_RS04745	987951..988208	+	258	WP_107220212.1	DUF4160 domain-containing protein	-
Tharo_RS04750	988177..988431	+	255	WP_107220213.1	DUF2442 domain-containing protein	-
Tharo_RS17720	988597..989751	+	1155	WP_211309657.1	hypothetical protein	-
Tharo_RS04760	989761..990288	+	528	WP_107220214.1	tetratricopeptide repeat protein	-
Tharo_RS04765	990603..990926	-	324	Protein_939	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
Tharo_RS04770	990913..991164	-	252	WP_107220215.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
Tharo_RS04775	991373..991696	+	324	WP_107220216.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Tharo_RS04780	991689..991979	+	291	WP_107220217.1	transcriptional regulator	Antitoxin
Tharo_RS04785	992077..992457	+	381	WP_107220218.1	clan AA aspartic protease	-
Tharo_RS04790	992464..992697	+	234	Protein_944	transposase	-
Tharo_RS04800	993220..993432	+	213	WP_107220219.1	hypothetical protein	-
Tharo_RS04805	993429..993700	+	272	Protein_946	type II toxin-antitoxin system RelE/ParE family toxin	-
Tharo_RS04810	993848..994105	+	258	WP_107220220.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
Tharo_RS04815	994095..994373	+	279	WP_107220221.1	Txe/YoeB family addiction module toxin	-
Tharo_RS17725	994490..994831	-	342	WP_211309658.1	hypothetical protein	-
Tharo_RS04825	994949..995551	+	603	WP_107220222.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-86)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11966.78 Da
• Isoelectric Point: 7.1315

>T100993 WP_107220216.1 NZ_CP028339:991373-991696 [Thauera aromatica K172]
MVFVELPIFVRCASELFSDDDLRELQVTLLENPAAGDLIPGGRGLRKLRAPLPGRGKRGGARVIYYYWVGHDQCYLVYAY
AKNAAADLTKDQLRRLAEVMDVEVSHG

Nucleotide

• Length: 324 bp

>T100993 NZ_CP028339:991373-991696 [Thauera aromatica K172]
ATGGTCTTTGTAGAACTTCCCATCTTCGTTCGTTGCGCCAGTGAGCTTTTTTCGGACGACGACTTGCGCGAGCTACAGGT
GACCTTGCTGGAGAATCCTGCTGCCGGTGATCTGATTCCTGGTGGTCGTGGTCTTCGCAAGTTGCGTGCGCCGTTACCTG
GGAGAGGAAAACGAGGGGGCGCAAGAGTGATCTACTACTATTGGGTTGGGCACGATCAGTGCTACTTGGTCTACGCCTAC
GCCAAGAATGCTGCGGCAGACCTGACCAAGGATCAGCTAAGGCGACTGGCCGAGGTGATGGATGTGGAGGTGAGTCATGG
ATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10840.48 Da
• Isoelectric Point: 10.2635

>AT100993 WP_107220217.1 NZ_CP028339:991689-991979 [Thauera aromatica K172]
MDDKQFDELLESVRDIGRHMRGEAVAGAKVREFPEPDVKAIRERTGLSQTRFAYLIGVKPKTLQNWEQHRVRPAGPARAL
LKIVEANPNALSVLHV

Nucleotide

• Length: 291 bp

>AT100993 NZ_CP028339:991689-991979 [Thauera aromatica K172]
ATGGATGACAAGCAATTTGACGAGCTGTTGGAGAGCGTTCGCGACATAGGGCGTCACATGCGTGGCGAGGCCGTTGCGGG
AGCGAAGGTGCGGGAGTTCCCGGAGCCTGACGTCAAGGCCATCCGGGAGCGCACAGGACTGAGCCAGACCCGTTTCGCCT
ACCTGATCGGGGTTAAACCAAAGACACTGCAGAATTGGGAGCAGCACCGGGTTCGCCCAGCAGGCCCGGCTCGTGCGCTA
CTGAAGATCGTCGAGGCCAACCCGAATGCCTTGTCGGTGCTTCACGTCTGA