Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2566149..2566711 Replicon chromosome
Accession NZ_CP028320
Organism Escherichia coli O18:H1 strain CFSAN067215

Toxin (Protein)


Gene name higB Uniprot ID A0A6G1XIR8
Locus tag C7X15_RS12830 Protein ID WP_048338064.1
Coordinates 2566433..2566711 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag C7X15_RS12825 Protein ID WP_000781370.1
Coordinates 2566149..2566433 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7X15_RS12810 2561330..2564377 + 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -
C7X15_RS12815 2564390..2565274 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
C7X15_RS12820 2565267..2565920 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
C7X15_RS12825 2566149..2566433 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
C7X15_RS12830 2566433..2566711 - 279 WP_048338064.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C7X15_RS12835 2566897..2567907 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
C7X15_RS12840 2568041..2569738 - 1698 WP_000433460.1 malate dehydrogenase -
C7X15_RS12845 2569895..2570032 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
C7X15_RS12850 2570134..2570349 - 216 WP_000495766.1 biofilm-dependent modulation protein -
C7X15_RS12855 2570694..2571125 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10540.18 Da        Isoelectric Point: 6.8582

>T100965 WP_048338064.1 NZ_CP028320:c2566711-2566433 [Escherichia coli O18:H1]
MIMNFRHMGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T100965 NZ_CP028320:c2566711-2566433 [Escherichia coli O18:H1]
ATGATAATGAATTTCAGACACATGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACTTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT100965 WP_000781370.1 NZ_CP028320:c2566433-2566149 [Escherichia coli O18:H1]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT100965 NZ_CP028320:c2566433-2566149 [Escherichia coli O18:H1]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G1XIR8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References