Detailed information of TA system TA100956

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 880874..881286
• Replicon: chromosome
• Accession: NZ_CP028320
• Organism: Escherichia coli O18:H1 strain CFSAN067215

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: C7X15_RS04295
• Protein ID: WP_000132630.1
• Coordinates: 880874..881215 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	881210..881286 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7X15_RS04275	875943..877223	-	1281	WP_001304526.1	DUF445 domain-containing protein	-
C7X15_RS04280	877408..878328	+	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
C7X15_RS04285	878556..878636	+	81	WP_020233658.1	hypothetical protein	-
C7X15_RS04290	878734..880827	+	2094	WP_000648234.1	DUF262 domain-containing protein	-
C7X15_RS04295	880874..881215	-	342	WP_000132630.1	endoribonuclease SymE	Toxin
-	881210..881286	+	77	NuclAT_6	-	Antitoxin
-	881210..881286	+	77	NuclAT_6	-	Antitoxin
-	881210..881286	+	77	NuclAT_6	-	Antitoxin
-	881210..881286	+	77	NuclAT_6	-	Antitoxin
-	881210..881286	+	77	NuclAT_7	-	Antitoxin
-	881210..881286	+	77	NuclAT_7	-	Antitoxin
-	881210..881286	+	77	NuclAT_7	-	Antitoxin
-	881210..881286	+	77	NuclAT_7	-	Antitoxin
C7X15_RS04300	881436..883205	-	1770	WP_000110076.1	restriction endonuclease subunit S	-
C7X15_RS04305	883205..884674	-	1470	WP_001315223.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T100956 WP_000132630.1 NZ_CP028320:c881215-880874 [Escherichia coli O18:H1]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T100956 NZ_CP028320:c881215-880874 [Escherichia coli O18:H1]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT100956 NZ_CP028320:881210-881286 [Escherichia coli O18:H1]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin