Detailed information of TA system TA100931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2942418..2943145
• Replicon: chromosome
• Accession: NZ_CP028273
• Organism: Mixta theicola strain SRCM103227

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C7M52_RS13490
• Protein ID: WP_160250742.1
• Coordinates: 2942834..2943145 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7M52_RS13485
• Protein ID: WP_160250741.1
• Coordinates: 2942418..2942837 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M52_RS13475	2939257..2941980	+	2724	WP_160250740.1	CRISPR-associated helicase Cas3'	-
C7M52_RS13480	2942141..2942326	+	186	WP_141175097.1	hypothetical protein	-
C7M52_RS13485	2942418..2942837	-	420	WP_160250741.1	helix-turn-helix domain-containing protein	Antitoxin
C7M52_RS13490	2942834..2943145	-	312	WP_160250742.1	type II toxin-antitoxin system HigB family toxin	Toxin
C7M52_RS13495	2945950..2947182	+	1233	WP_160251362.1	imidazolonepropionase	-
C7M52_RS13500	2947179..2948132	+	954	WP_160250743.1	formimidoylglutamase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12285.07 Da
• Isoelectric Point: 10.0729

>T100931 WP_160250742.1 NZ_CP028273:c2943145-2942834 [Mixta theicola]
MHVISKEPFEQAARSFPNDATAIKALYRLVRETDFKTPEELRAAVPSLDNFKYRKKWWVLDVGGSNLRVIAYINFVNKRF
YVKHIATHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T100931 NZ_CP028273:c2943145-2942834 [Mixta theicola]
ATGCACGTCATTTCAAAAGAGCCATTTGAGCAAGCGGCGCGGTCATTTCCTAACGATGCTACGGCCATCAAGGCACTGTA
TCGTCTGGTACGCGAAACAGACTTCAAAACGCCCGAAGAACTGCGCGCCGCCGTGCCAAGCCTGGACAATTTTAAGTACC
GTAAAAAATGGTGGGTTTTAGATGTAGGGGGAAGCAATTTGCGAGTGATCGCCTACATCAACTTCGTGAATAAGCGCTTC
TACGTGAAGCACATCGCCACCCACGCTGAATACGATAAGCTGACTCGCTACTATCGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15454.61 Da
• Isoelectric Point: 4.5365

>AT100931 WP_160250741.1 NZ_CP028273:c2942837-2942418 [Mixta theicola]
MIADTTKAIEATKLLVAAVPLLGGSTSEKDYRDALALMDYLIDHDDENPLIDFLAAKIADYEDNSERFAEFNKEVAEMPV
GVALLRTLIDQHQLSYSDLKNEIGSKSLVSQILSGQRSLTISHIKALSERFGVKPQWFL

Nucleotide

• Length: 420 bp

>AT100931 NZ_CP028273:c2942837-2942418 [Mixta theicola]
ATGATCGCCGACACCACTAAAGCTATCGAAGCAACGAAACTACTGGTAGCTGCCGTCCCCCTGCTGGGGGGCAGCACCTC
AGAGAAAGACTACCGTGACGCGCTGGCGCTGATGGACTACCTGATTGACCACGACGACGAAAACCCGCTGATCGACTTCC
TGGCGGCCAAAATTGCCGACTACGAAGACAACAGCGAACGTTTTGCGGAGTTCAACAAAGAAGTCGCAGAAATGCCGGTG
GGCGTCGCGTTGCTGCGCACGCTGATTGATCAGCACCAGCTGTCATACTCCGACCTGAAGAATGAGATCGGCTCTAAATC
GCTGGTGAGCCAGATCCTCTCCGGTCAGCGCTCCCTGACGATCTCACACATCAAGGCGCTTTCGGAACGGTTCGGCGTGA
AGCCGCAATGGTTCCTGTAA