Detailed information of TA system TA100840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 12914..13560
• Replicon: plasmid pGMI14-004_3
• Accession: NZ_CP028194
• Organism: Escherichia coli strain CFSAN018748

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C7A99_RS26590
• Protein ID: WP_000269912.1
• Coordinates: 12914..13261 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7A99_RS26595
• Protein ID: WP_001673379.1
• Coordinates: 13261..13560 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A99_RS26550	8052..8654	-	603	WP_107236354.1	tail fiber assembly protein	-
C7A99_RS26555	8654..9082	-	429	WP_107236355.1	phage tail protein	-
C7A99_RS26560	9110..9640	-	531	WP_107236356.1	phage tail protein	-
C7A99_RS26565	9657..10217	+	561	WP_107236357.1	recombinase family protein	-
C7A99_RS26570	10615..11451	+	837	WP_107236358.1	RepA	-
C7A99_RS26580	12095..12382	+	288	WP_000356589.1	hypothetical protein	-
C7A99_RS26585	12406..12669	+	264	WP_000424604.1	hypothetical protein	-
C7A99_RS26590	12914..13261	+	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7A99_RS26595	13261..13560	+	300	WP_001673379.1	XRE family transcriptional regulator	Antitoxin
C7A99_RS26600	13594..13878	-	285	WP_021542820.1	hypothetical protein	-
C7A99_RS26605	14146..14526	+	381	WP_000061763.1	hypothetical protein	-
C7A99_RS26610	14590..14862	+	273	WP_000148348.1	helix-turn-helix domain-containing protein	-
C7A99_RS26615	15498..16133	+	636	WP_027662196.1	hypothetical protein	-
C7A99_RS26620	16168..17310	-	1143	WP_107236360.1	ORF6N domain-containing protein	-
C7A99_RS26625	17307..17498	-	192	WP_000183351.1	hypothetical protein	-
C7A99_RS26630	17609..17803	-	195	WP_157836022.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48684	48684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T100840 WP_000269912.1 NZ_CP028194:12914-13261 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T100840 NZ_CP028194:12914-13261 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11069.90 Da
• Isoelectric Point: 5.8899

>AT100840 WP_001673379.1 NZ_CP028194:13261-13560 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT100840 NZ_CP028194:13261-13560 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCTGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCTACAGGAAAGCGAGTAGCGTTCCATGTCTAA