Detailed information of TA system TA100833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4398369..4399063
• Replicon: chromosome
• Accession: NZ_CP028192
• Organism: Escherichia coli strain CFSAN018748

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: C7A99_RS22760
• Protein ID: WP_107236192.1
• Coordinates: 4398369..4398767 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: C7A99_RS22765
• Protein ID: WP_107236193.1
• Coordinates: 4398770..4399063 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A99_RS22730	4393539..4394783	-	1245	WP_107236189.1	esterase FrsA	-
-	4394199..4394279	-	81	NuclAT_10	-	-
-	4394199..4394279	-	81	NuclAT_10	-	-
-	4394199..4394279	-	81	NuclAT_10	-	-
-	4394199..4394279	-	81	NuclAT_10	-	-
C7A99_RS22735	4394875..4395333	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C7A99_RS22740	4395594..4397051	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
C7A99_RS22745	4397180..4397460	-	281	Protein_4145	peptide chain release factor H	-
C7A99_RS22750	4397456..4397662	-	207	Protein_4146	RtcB family protein	-
C7A99_RS22755	4397907..4398359	-	453	WP_107236191.1	GNAT family N-acetyltransferase	-
C7A99_RS22760	4398369..4398767	-	399	WP_107236192.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C7A99_RS22765	4398770..4399063	-	294	WP_107236193.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C7A99_RS22770	4399115..4400170	-	1056	WP_001226164.1	DNA polymerase IV	-
C7A99_RS22775	4400241..4401026	-	786	WP_199852819.1	putative lateral flagellar export/assembly protein LafU	-
C7A99_RS22780	4400998..4402710	+	1713	Protein_4152	flagellar biosynthesis protein FlhA	-
C7A99_RS22785	4402815..4403093	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
C7A99_RS22790	4403086..4403442	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4370653..4406027	35374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15414.83 Da
• Isoelectric Point: 7.4213

>T100833 WP_107236192.1 NZ_CP028192:c4398767-4398369 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMLF

Nucleotide

• Length: 399 bp

>T100833 NZ_CP028192:c4398767-4398369 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCTTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11232.78 Da
• Isoelectric Point: 5.8944

>AT100833 WP_107236193.1 NZ_CP028192:c4399063-4398770 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNNFKE

Nucleotide

• Length: 294 bp

>AT100833 NZ_CP028192:c4399063-4398770 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCTAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATAACTTTAAGGAATAA