Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2380030..2380532 Replicon chromosome
Accession NZ_CP028192
Organism Escherichia coli strain CFSAN018748

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7A99_RS12040 Protein ID WP_000767829.1
Coordinates 2380278..2380532 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C7A99_RS12035 Protein ID WP_001259255.1
Coordinates 2380030..2380281 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A99_RS12005 2375208..2376275 - 1068 WP_000080111.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C7A99_RS12010 2376275..2377345 - 1071 WP_063085464.1 histidinol-phosphate transaminase -
C7A99_RS12015 2377342..2378646 - 1305 WP_063085463.1 histidinol dehydrogenase -
C7A99_RS12020 2378652..2379551 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C7A99_RS12030 2379697..2379747 - 51 WP_001364200.1 his operon leader peptide -
C7A99_RS12035 2380030..2380281 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7A99_RS12040 2380278..2380532 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7A99_RS12045 2380615..2381439 + 825 WP_000754737.1 SDR family oxidoreductase -
C7A99_RS12050 2381485..2382414 + 930 WP_000803351.1 LysR family transcriptional regulator -
C7A99_RS12055 2382629..2382691 + 63 WP_010723108.1 membrane protein YoeI -
C7A99_RS12060 2382681..2384039 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7A99_RS12070 2384253..2384711 + 459 WP_000526135.1 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2384253..2384711 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T100820 WP_000767829.1 NZ_CP028192:2380278-2380532 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T100820 NZ_CP028192:2380278-2380532 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT100820 WP_001259255.1 NZ_CP028192:2380030-2380281 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT100820 NZ_CP028192:2380030-2380281 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References