Detailed information of TA system TA100819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1615525..1616252
• Replicon: chromosome
• Accession: NZ_CP028192
• Organism: Escherichia coli strain CFSAN018748

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: C7A99_RS08290
• Protein ID: WP_000547555.1
• Coordinates: 1615525..1615836 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7A99_RS08295
• Protein ID: WP_000126294.1
• Coordinates: 1615833..1616252 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A99_RS08265	1611460..1613169	+	1710	WP_107235693.1	formate hydrogenlyase subunit HycE	-
C7A99_RS08270	1613179..1613721	+	543	WP_107235694.1	formate hydrogenlyase subunit HycF	-
C7A99_RS08275	1613721..1614488	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
C7A99_RS08280	1614485..1614895	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
C7A99_RS08285	1614888..1615358	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C7A99_RS08290	1615525..1615836	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
C7A99_RS08295	1615833..1616252	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
C7A99_RS08300	1616331..1617755	-	1425	WP_063086210.1	6-phospho-beta-glucosidase AscB	-
C7A99_RS08305	1617764..1619221	-	1458	WP_107235695.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C7A99_RS08310	1619481..1620491	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
C7A99_RS08315	1620640..1621167	+	528	WP_107235696.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T100819 WP_000547555.1 NZ_CP028192:1615525-1615836 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T100819 NZ_CP028192:1615525-1615836 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT100819 WP_000126294.1 NZ_CP028192:1615833-1616252 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT100819 NZ_CP028192:1615833-1616252 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGATACTAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAATAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2