Detailed information of TA system TA100814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 220895..221497
• Replicon: chromosome
• Accession: NZ_CP028192
• Organism: Escherichia coli strain CFSAN018748

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C7A99_RS01190
• Protein ID: WP_000897305.1
• Coordinates: 221186..221497 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7A99_RS01185
• Protein ID: WP_107235477.1
• Coordinates: 220895..221185 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A99_RS01145	216518..217420	+	903	WP_107235476.1	formate dehydrogenase O subunit beta	-
C7A99_RS01150	217417..218052	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C7A99_RS01155	218049..218978	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C7A99_RS01160	219160..219402	-	243	WP_001306649.1	hypothetical protein	-
C7A99_RS01165	219621..219839	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C7A99_RS01175	220258..220536	-	279	WP_001306650.1	hypothetical protein	-
C7A99_RS01180	220598..220810	-	213	WP_000197769.1	hypothetical protein	-
C7A99_RS01185	220895..221185	-	291	WP_107235477.1	helix-turn-helix domain-containing protein	Antitoxin
C7A99_RS01190	221186..221497	-	312	WP_000897305.1	hypothetical protein	Toxin
C7A99_RS01195	221726..222634	+	909	WP_001306651.1	alpha/beta hydrolase	-
C7A99_RS01205	222803..224617	-	1815	WP_107235478.1	hypothetical protein	-
C7A99_RS01210	225033..225974	-	942	WP_159042843.1	fatty acid biosynthesis protein FabY	-
C7A99_RS01215	226019..226456	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T100814 WP_000897305.1 NZ_CP028192:c221497-221186 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T100814 NZ_CP028192:c221497-221186 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10850.42 Da
• Isoelectric Point: 10.5848

>AT100814 WP_107235477.1 NZ_CP028192:c221185-220895 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRHASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT100814 NZ_CP028192:c221185-220895 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCACGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7