Detailed information of TA system TA100755

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 38068..38332
• Replicon: plasmid pGMI17-004_1
• Accession: NZ_CP028168
• Organism: Escherichia coli strain CFSAN064036

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: C6668_RS25485
• Protein ID: WP_001331364.1
• Coordinates: 38068..38220 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	38275..38332 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6668_RS25465	33346..35508	+	2163	WP_000698351.1	DotA/TraY family protein	-
C6668_RS25470	35573..36235	+	663	WP_000653334.1	plasmid IncI1-type surface exclusion protein ExcA	-
C6668_RS25475	36307..36516	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
C6668_RS26450	36908..37084	+	177	WP_001054904.1	hypothetical protein	-
C6668_RS25480	37149..37445	-	297	WP_001275298.1	hypothetical protein	-
C6668_RS25485	38068..38220	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	38275..38332	+	58	NuclAT_0	-	Antitoxin
-	38275..38332	+	58	NuclAT_0	-	Antitoxin
-	38275..38332	+	58	NuclAT_0	-	Antitoxin
-	38275..38332	+	58	NuclAT_0	-	Antitoxin
C6668_RS25490	38512..39720	+	1209	WP_000121274.1	IncI1-type conjugal transfer protein TrbA	-
C6668_RS25495	39739..40809	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
C6668_RS25500	40802..43093	+	2292	WP_001289276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sul1 / qacE / ant(3'')-Ia / dfrA1 / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A)	-	1..119076	119076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T100755 WP_001331364.1 NZ_CP028168:c38220-38068 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T100755 NZ_CP028168:c38220-38068 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT100755 NZ_CP028168:38275-38332 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin