Detailed information of TA system TA100750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4228167..4228708
• Replicon: chromosome
• Accession: NZ_CP028166
• Organism: Escherichia coli strain CFSAN064036

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q32JN3
• Locus tag: C6668_RS22015
• Protein ID: WP_000615980.1
• Coordinates: 4228167..4228445 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: C6668_RS22020
• Protein ID: WP_000729707.1
• Coordinates: 4228448..4228708 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6668_RS22000	4225749..4226327	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
C6668_RS22005	4226533..4227300	+	768	WP_000333380.1	class II glutamine amidotransferase	-
C6668_RS22010	4227271..4228011	-	741	WP_001225679.1	murein L,D-transpeptidase	-
C6668_RS22015	4228167..4228445	-	279	WP_000615980.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
C6668_RS22020	4228448..4228708	-	261	WP_000729707.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
C6668_RS22025	4228894..4229667	+	774	WP_000543885.1	C40 family peptidase	-
C6668_RS22030	4229843..4230340	+	498	WP_000006245.1	REP-associated tyrosine transposase RayT	-
C6668_RS22035	4230472..4232184	-	1713	Protein_4036	flagellar biosynthesis protein FlhA	-
C6668_RS22040	4232156..4232941	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T100750 WP_000615980.1 NZ_CP028166:c4228445-4228167 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T100750 NZ_CP028166:c4228445-4228167 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9421.80 Da
• Isoelectric Point: 5.0758

>AT100750 WP_000729707.1 NZ_CP028166:c4228708-4228448 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKTKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT100750 NZ_CP028166:c4228708-4228448 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGACCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A090NH48