Detailed information of TA system TA100747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2520270..2521069
• Replicon: chromosome
• Accession: NZ_CP028166
• Organism: Escherichia coli strain CFSAN064036

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: C6668_RS13300
• Protein ID: WP_000347273.1
• Coordinates: 2520605..2521069 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: C6668_RS13295
• Protein ID: WP_001307405.1
• Coordinates: 2520270..2520605 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6668_RS13280	2516055..2516825	-	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-
C6668_RS13285	2516841..2518175	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
C6668_RS13290	2518550..2520121	+	1572	WP_001273753.1	galactarate dehydratase	-
C6668_RS13295	2520270..2520605	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C6668_RS13300	2520605..2521069	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C6668_RS13305	2521124..2521933	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C6668_RS13315	2522182..2523462	+	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C6668_RS13320	2523485..2523958	+	474	WP_001336162.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C6668_RS13325	2523969..2524748	+	780	WP_000406209.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C6668_RS13330	2524738..2525616	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C6668_RS13335	2525634..2526068	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T100747 WP_000347273.1 NZ_CP028166:2520605-2521069 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T100747 NZ_CP028166:2520605-2521069 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT100747 WP_001307405.1 NZ_CP028166:2520270-2520605 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT100747 NZ_CP028166:2520270-2520605 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7