Detailed information of TA system TA100727

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 585004..585375
• Replicon: chromosome
• Accession: NZ_CP028166
• Organism: Escherichia coli strain CFSAN064036

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: C6668_RS03190
• Protein ID: WP_001317028.1
• Coordinates: 585181..585375 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	585004..585182 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6668_RS03165	580959..582332	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
C6668_RS03170	582461..583396	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C6668_RS03175	583448..584683	-	1236	WP_000040852.1	site-specific integrase	-
C6668_RS03180	584685..584900	-	216	WP_000079604.1	excisionase XisR	-
C6668_RS03185	584979..585188	-	210	WP_032234345.1	double-strand break reduction protein RcbA	-
-	585004..585182	+	179	NuclAT_0	-	Antitoxin
-	585004..585182	+	179	NuclAT_0	-	Antitoxin
-	585004..585182	+	179	NuclAT_0	-	Antitoxin
-	585004..585182	+	179	NuclAT_0	-	Antitoxin
C6668_RS03190	585181..585375	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C6668_RS03195	585432..586241	-	810	WP_000166319.1	recombination protein RecT	-
C6668_RS03200	586234..588834	-	2601	WP_000105124.1	exodeoxyribonuclease VIII	-
C6668_RS03205	588936..589211	-	276	WP_000632297.1	protein RacC	-
C6668_RS03210	589286..589456	-	171	WP_001352098.1	conserved protein, Rac prophage	-
C6668_RS03215	589456..589677	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	576189..601706	25517

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T100727 WP_001317028.1 NZ_CP028166:c585375-585181 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T100727 NZ_CP028166:c585375-585181 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT100727 NZ_CP028166:585004-585182 [Escherichia coli]
GAGGACTGAAGTTTCGCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin