Detailed information of TA system TA100682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 7265..7857
• Replicon: plasmid pVpR14_20Kb
• Accession: NZ_CP028143
• Organism: Vibrio parahaemolyticus strain R14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C9I78_RS25905
• Protein ID: WP_080608540.1
• Coordinates: 7540..7857 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C9I78_RS25900
• Protein ID: WP_080608539.1
• Coordinates: 7265..7543 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9I78_RS25875	3695..4252	+	558	WP_031419876.1	recombinase family protein	-
C9I78_RS25880	4657..5535	-	879	WP_108745456.1	tyrosine-type recombinase/integrase	-
C9I78_RS25885	5758..6132	-	375	WP_080608536.1	hypothetical protein	-
C9I78_RS25890	6129..6644	-	516	WP_080608537.1	ParA family protein	-
C9I78_RS25895	6851..7225	+	375	WP_108745457.1	DUF4236 domain-containing protein	-
C9I78_RS25900	7265..7543	-	279	WP_080608539.1	helix-turn-helix transcriptional regulator	Antitoxin
C9I78_RS25905	7540..7857	-	318	WP_080608540.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9I78_RS25915	8790..9674	+	885	WP_108745458.1	site-specific integrase	-
C9I78_RS25920	9671..10330	+	660	WP_108745459.1	hypothetical protein	-
C9I78_RS25925	10379..10771	+	393	WP_108745460.1	hypothetical protein	-
C9I78_RS26765	10774..10941	+	168	WP_159087269.1	hypothetical protein	-
C9I78_RS25930	11001..11288	+	288	WP_080608545.1	hypothetical protein	-
C9I78_RS25935	11329..12351	-	1023	WP_103199658.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..19217	19217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-86)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12017.19 Da
• Isoelectric Point: 9.8772

>T100682 WP_080608540.1 NZ_CP028143:c7857-7540 [Vibrio parahaemolyticus]
MAWKVDFHDGVEDEILSMPPKIQARMIKLLELIEMHGANLGPPHTEAMGDGLFEIRAKAKEGIGRGLFCYLKGQHVYVLR
AFVKKSNKTPKKEIELARERMREVK

Nucleotide

• Length: 318 bp

>T100682 NZ_CP028143:c7857-7540 [Vibrio parahaemolyticus]
ATGGCTTGGAAAGTAGATTTTCATGATGGTGTTGAGGATGAAATCCTATCAATGCCGCCAAAAATCCAAGCACGTATGAT
TAAGCTATTAGAGCTGATCGAGATGCATGGGGCCAACTTGGGACCGCCGCATACAGAAGCAATGGGTGATGGTCTTTTTG
AGATCAGAGCTAAAGCAAAAGAGGGGATCGGTCGAGGTTTGTTTTGTTATCTAAAGGGGCAACATGTCTATGTTCTTAGG
GCCTTTGTGAAGAAGTCTAACAAAACACCAAAGAAAGAGATCGAACTGGCCCGAGAAAGAATGAGAGAGGTGAAATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10183.72 Da
• Isoelectric Point: 8.0019

>AT100682 WP_080608539.1 NZ_CP028143:c7543-7265 [Vibrio parahaemolyticus]
MMSLAALKKKAFTNQEVKEAYDELGDEFELISTLITMREKSGLTQDEVAQKMGTRAPNISRLESGRGNPSLKTLVSYAQA
CGFKLDLGFKHV

Nucleotide

• Length: 279 bp

>AT100682 NZ_CP028143:c7543-7265 [Vibrio parahaemolyticus]
ATGATGAGCTTAGCTGCATTGAAAAAGAAAGCTTTTACCAATCAAGAGGTGAAAGAAGCGTACGATGAGCTTGGTGATGA
ATTTGAGCTCATTAGTACTCTGATCACGATGAGAGAAAAATCAGGACTGACTCAGGATGAAGTTGCCCAAAAAATGGGAA
CCCGAGCTCCTAATATTTCTCGCCTAGAGTCAGGTAGAGGAAACCCTAGCTTAAAAACCCTCGTTAGCTATGCTCAAGCG
TGTGGCTTCAAGTTAGACCTTGGCTTTAAGCATGTTTGA