Detailed information of TA system TA100679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 51644..52295
• Replicon: plasmid pAbNCIMB8209_134
• Accession: NZ_CP028139
• Organism: Acinetobacter baumannii strain NCIMB 8209

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7H2WXC0
• Locus tag: C4X49_RS18740
• Protein ID: WP_006581604.1
• Coordinates: 51644..52000 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4X49_RS18745
• Protein ID: WP_001140619.1
• Coordinates: 51993..52295 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4X49_RS18710	47530..47754	+	225	WP_125510319.1	hypothetical protein	-
C4X49_RS18715	47788..48150	+	363	Protein_50	plasmid replication DNA-binding protein	-
C4X49_RS18720	48350..49171	-	822	WP_017725646.1	GIY-YIG nuclease family protein	-
C4X49_RS18725	49363..49665	+	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4X49_RS18730	49666..49947	+	282	WP_000985610.1	putative addiction module antidote protein	-
C4X49_RS18735	50151..51023	+	873	WP_006581603.1	restriction endonuclease	-
C4X49_RS18740	51644..52000	+	357	WP_006581604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4X49_RS18745	51993..52295	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
C4X49_RS18750	52288..52497	+	210	WP_000069471.1	hypothetical protein	-
C4X49_RS18755	52607..52825	-	219	WP_029747731.1	hypothetical protein	-
C4X49_RS18770	54822..55367	-	546	Protein_60	IS5 family transposase	-
C4X49_RS18775	55295..55683	-	389	Protein_61	AAA family ATPase	-
C4X49_RS18780	55869..56189	+	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
C4X49_RS18785	56466..56654	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4X49_RS18790	56641..56930	+	290	Protein_64	helix-turn-helix domain-containing protein	-
C4X49_RS18795	57009..57263	-	255	WP_000236586.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..133709	133709
	-	flank	IS/Tn	-	-	46085..47293	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13462.38 Da
• Isoelectric Point: 7.1642

>T100679 WP_006581604.1 NZ_CP028139:51644-52000 [Acinetobacter baumannii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKDLRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T100679 NZ_CP028139:51644-52000 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGACCTACGTGTTCAGCATCGAGGTAAATCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGTGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT100679 WP_001140619.1 NZ_CP028139:51993-52295 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT100679 NZ_CP028139:51993-52295 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAATTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A7H2WXC0