Detailed information of TA system TA100658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5213706..5214308
• Replicon: chromosome
• Accession: NZ_CP028126
• Organism: Escherichia coli O26 str. RM10386

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I3U_RS29675
• Protein ID: WP_000897305.1
• Coordinates: 5213706..5214017 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I3U_RS29680
• Protein ID: WP_000356397.1
• Coordinates: 5214018..5214308 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U_RS29645	5208736..5209521	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
I3U_RS29650	5209620..5210219	+	600	WP_001315111.1	glucose-1-phosphatase	-
I3U_RS29655	5210213..5211085	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I3U_RS29660	5211082..5211519	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I3U_RS29665	5211564..5212505	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I3U_RS29670	5212569..5213477	-	909	WP_001162704.1	alpha/beta hydrolase	-
I3U_RS29675	5213706..5214017	+	312	WP_000897305.1	hypothetical protein	Toxin
I3U_RS29680	5214018..5214308	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I3U_RS29690	5214913..5215131	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
I3U_RS29695	5215350..5215592	+	243	WP_001086390.1	hypothetical protein	-
I3U_RS29700	5215821..5216801	-	981	WP_000399685.1	IS110-like element IS621 family transposase	-
I3U_RS29710	5217065..5218168	-	1104	WP_000374004.1	bifunctional L-1,2-propanediol dehydrogenase/glycerol dehydrogenase	-
I3U_RS29715	5218179..5218841	-	663	WP_000424845.1	fructose-6-phosphate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5215821..5216801	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T100658 WP_000897305.1 NZ_CP028126:5213706-5214017 [Escherichia coli O26 str. RM10386]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T100658 NZ_CP028126:5213706-5214017 [Escherichia coli O26 str. RM10386]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT100658 WP_000356397.1 NZ_CP028126:5214018-5214308 [Escherichia coli O26 str. RM10386]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT100658 NZ_CP028126:5214018-5214308 [Escherichia coli O26 str. RM10386]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7