Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2863089..2863591 Replicon chromosome
Accession NZ_CP028126
Organism Escherichia coli O26 str. RM10386

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag I3U_RS17295 Protein ID WP_000767829.1
Coordinates 2863089..2863343 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I3U_RS17300 Protein ID WP_001259255.1
Coordinates 2863340..2863591 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U_RS17265 2858104..2858331 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
I3U_RS17270 2858345..2859403 - 1059 WP_000492324.1 transport protein YeeE -
I3U_RS17275 2859582..2860940 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I3U_RS17280 2860930..2860992 - 63 WP_010723108.1 membrane protein YoeI -
I3U_RS17285 2861207..2862136 - 930 WP_000803351.1 LysR family transcriptional regulator -
I3U_RS17290 2862182..2863006 - 825 WP_000754737.1 SDR family oxidoreductase -
I3U_RS17295 2863089..2863343 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I3U_RS17300 2863340..2863591 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I3U_RS17305 2863874..2863921 + 48 WP_001375657.1 his operon leader peptide -
I3U_RS17315 2864067..2864966 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
I3U_RS17320 2864972..2866276 + 1305 WP_000009589.1 histidinol dehydrogenase -
I3U_RS17325 2866273..2867343 + 1071 WP_000108914.1 histidinol-phosphate transaminase -
I3U_RS17330 2867343..2868410 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T100652 WP_000767829.1 NZ_CP028126:c2863343-2863089 [Escherichia coli O26 str. RM10386]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T100652 NZ_CP028126:c2863343-2863089 [Escherichia coli O26 str. RM10386]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT100652 WP_001259255.1 NZ_CP028126:c2863591-2863340 [Escherichia coli O26 str. RM10386]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT100652 NZ_CP028126:c2863591-2863340 [Escherichia coli O26 str. RM10386]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References