Detailed information of TA system TA100635

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1924878..1925249
• Replicon: chromosome
• Accession: NZ_CP028126
• Organism: Escherichia coli O26 str. RM10386

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: I3U_RS11990
• Protein ID: WP_001443846.1
• Coordinates: 1925100..1925249 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1924878..1925056 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U_RS11965	1920833..1922206	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
I3U_RS11970	1922335..1923270	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
I3U_RS11975	1923322..1924557	-	1236	WP_000040851.1	site-specific integrase	-
I3U_RS11980	1924559..1924774	-	216	WP_000079604.1	excisionase XisR	-
I3U_RS11985	1924874..1925062	-	189	WP_001302840.1	DUF1187 family protein	-
-	1924878..1925056	+	179	NuclAT_0	-	Antitoxin
-	1924878..1925056	+	179	NuclAT_0	-	Antitoxin
-	1924878..1925056	+	179	NuclAT_0	-	Antitoxin
-	1924878..1925056	+	179	NuclAT_0	-	Antitoxin
I3U_RS11990	1925100..1925249	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
I3U_RS11995	1925305..1926114	-	810	WP_000166313.1	recombination protein RecT	-
I3U_RS12000	1926107..1928707	-	2601	WP_000105151.1	exodeoxyribonuclease VIII	-
I3U_RS12005	1928809..1929084	-	276	WP_000632297.1	protein RacC	-
I3U_RS12010	1929159..1929329	-	171	WP_001352098.1	conserved protein, Rac prophage	-
I3U_RS12015	1929329..1929550	-	222	WP_000560223.1	killing protein KilR	-
I3U_RS12020	1929971..1930123	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1916063..1969308	53245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T100635 WP_001443846.1 NZ_CP028126:c1925249-1925100 [Escherichia coli O26 str. RM10386]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T100635 NZ_CP028126:c1925249-1925100 [Escherichia coli O26 str. RM10386]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT100635 NZ_CP028126:1924878-1925056 [Escherichia coli O26 str. RM10386]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin